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-Structure paper
タイトル | Structural basis of TFIIIC-dependent RNA polymerase III transcription initiation. |
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ジャーナル・号・ページ | Mol Cell, Vol. 83, Issue 15, Page 2641-22652.e7, Year 2023 |
掲載日 | 2023年8月3日 |
![]() | Anna Talyzina / Yan Han / Chiranjib Banerjee / Susan Fishbain / Alexis Reyes / Reza Vafabakhsh / Yuan He / ![]() |
PubMed 要旨 | RNA polymerase III (Pol III) is responsible for transcribing 5S ribosomal RNA (5S rRNA), tRNAs, and other short non-coding RNAs. Its recruitment to the 5S rRNA promoter requires transcription factors ...RNA polymerase III (Pol III) is responsible for transcribing 5S ribosomal RNA (5S rRNA), tRNAs, and other short non-coding RNAs. Its recruitment to the 5S rRNA promoter requires transcription factors TFIIIA, TFIIIC, and TFIIIB. Here, we use cryoelectron microscopy (cryo-EM) to visualize the S. cerevisiae complex of TFIIIA and TFIIIC bound to the promoter. Gene-specific factor TFIIIA interacts with DNA and acts as an adaptor for TFIIIC-promoter interactions. We also visualize DNA binding of TFIIIB subunits, Brf1 and TBP (TATA-box binding protein), which results in the full-length 5S rRNA gene wrapping around the complex. Our smFRET study reveals that the DNA within the complex undergoes both sharp bending and partial dissociation on a slow timescale, consistent with the model predicted from our cryo-EM results. Our findings provide new insights into the transcription initiation complex assembly on the 5S rRNA promoter and allow us to directly compare Pol III and Pol II transcription adaptations. |
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手法 | EM (単粒子) |
解像度 | 3.8 - 7.14 Å |
構造データ | EMDB-29071, PDB-8ffz: ![]() EMDB-29356: Focused refinement on the Brf1-TBP-DNA within TFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene ![]() EMDB-29358: TFIIIA-TFIIIC complex bound to 5S rRNA gene |
化合物 | ![]() ChemComp-ZN: |
由来 |
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![]() | TRANSCRIPTION/DNA / transcription factor / TRANSCRIPTION / TRANSCRIPTION-DNA complex |