EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of BIRC6-client complexes provide a mechanism of SMAC-mediated release of caspases.
ジャーナル・号・ページ	Science, Vol. 379, Issue 6637, Page 1105-1111, Year 2023
掲載日	2023年3月17日
著者	Moritz Hunkeler / Cyrus Y Jin / Eric S Fischer /
PubMed 要旨	Tight regulation of apoptosis is essential for metazoan development and prevents diseases such as cancer and neurodegeneration. Caspase activation is central to apoptosis, and inhibitor of apoptosis ...Tight regulation of apoptosis is essential for metazoan development and prevents diseases such as cancer and neurodegeneration. Caspase activation is central to apoptosis, and inhibitor of apoptosis proteins (IAPs) are the principal actors that restrain caspase activity and are therefore attractive therapeutic targets. IAPs, in turn, are regulated by mitochondria-derived proapoptotic factors such as SMAC and HTRA2. Through a series of cryo-electron microscopy structures of full-length human baculoviral IAP repeat-containing protein 6 (BIRC6) bound to SMAC, caspases, and HTRA2, we provide a molecular understanding for BIRC6-mediated caspase inhibition and its release by SMAC. The architecture of BIRC6, together with near-irreversible binding of SMAC, elucidates how the IAP inhibitor SMAC can effectively control a processive ubiquitin ligase to respond to apoptotic stimuli.
リンク	Science / PubMed:36758104
手法	EM (単粒子)
解像度	1.98 - 3.44 Å
構造データ	27832 8e2d EMDB-27832, PDB-8e2d: Cryo-EM structure of BIRC6 (consensus) 手法: EM (単粒子) / 解像度: 2.07 Å 27833 8e2e EMDB-27833, PDB-8e2e: Cryo-EM structure of helical arch of BIRC6 (from local refinement 1) 手法: EM (単粒子) / 解像度: 1.98 Å 27834 8e2f EMDB-27834, PDB-8e2f: Cryo-EM structure of N-terminal arm of BIRC6 (from local refinement 2) 手法: EM (単粒子) / 解像度: 2.47 Å 27835 8e2g EMDB-27835, PDB-8e2g: Cryo-EM structure of N-terminal arm (aa68-966) of BIRC6 (from local refinement 3) 手法: EM (単粒子) / 解像度: 2.99 Å 27836 8e2h EMDB-27836, PDB-8e2h: Cryo-EM structure of C-terminal arm of BIRC6 (from local refinement 4) 手法: EM (単粒子) / 解像度: 2.3 Å 27837 8e2i EMDB-27837, PDB-8e2i: Cryo-EM structure of BIRC6/Smac 手法: EM (単粒子) / 解像度: 3.04 Å 27838 8e2j EMDB-27838, PDB-8e2j: Cryo-EM structure of BIRC6/Smac (from local refinement 1) 手法: EM (単粒子) / 解像度: 3.44 Å EMDB-27839: Cryo-EM density of BIRC6/casp-3 (clusters) 手法: EM (単粒子) / 解像度: 2.82 Å EMDB-27840: Cryo-EM density of BIRC6/casp-7 (clusters) 手法: EM (単粒子) / 解像度: 3.01 Å 27841 8e2k EMDB-27841, PDB-8e2k: Cryo-EM structure of BIRC6/HtrA2-S306A 手法: EM (単粒子) / 解像度: 3.21 Å
化合物	ChemComp-HOH: WATER
由来	homo sapiens (ヒト)
キーワード	LIGASE / Ubiquitin / E3 ligase / Apoptosis / Autophagy / IAP