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-Structure paper
タイトル | The lipid linked oligosaccharide polymerase Wzy and its regulating co-polymerase, Wzz, from enterobacterial common antigen biosynthesis form a complex. |
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ジャーナル・号・ページ | Open Biol, Vol. 13, Issue 3, Page 220373, Year 2023 |
掲載日 | 2023年3月22日 |
![]() | Miriam Weckener / Laura S Woodward / Bradley R Clarke / Huanting Liu / Philip N Ward / Audrey Le Bas / David Bhella / Chris Whitfield / James H Naismith / ![]() ![]() |
PubMed 要旨 | The enterobacterial common antigen (ECA) is a carbohydrate polymer that is associated with the cell envelope in the . ECA contains a repeating trisaccharide which is polymerized by WzyE, a member of ...The enterobacterial common antigen (ECA) is a carbohydrate polymer that is associated with the cell envelope in the . ECA contains a repeating trisaccharide which is polymerized by WzyE, a member of the Wzy membrane protein polymerase superfamily. WzyE activity is regulated by a membrane protein polysaccharide co-polymerase, WzzE. Förster resonance energy transfer experiments demonstrate that WzyE and WzzE from form a complex , and immunoblotting and cryo-electron microscopy (cryo-EM) analysis confirm a defined stoichiometry of approximately eight WzzE to one WzyE. Low-resolution cryo-EM reconstructions of the complex, aided by an antibody recognizing the C-terminus of WzyE, reveals WzyE sits in the central membrane lumen formed by the octameric arrangement of the transmembrane helices of WzzE. The pairing of Wzy and Wzz is found in polymerization systems for other bacterial polymers, including lipopolysaccharide O-antigens and capsular polysaccharides. The data provide new structural insight into a conserved mechanism for regulating polysaccharide chain length in bacteria. |
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手法 | EM (単粒子) |
解像度 | 3.6 Å |
構造データ | EMDB-16364, PDB-8c0e: |
由来 |
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![]() | MEMBRANE PROTEIN / enterobacterial common antigen / lipopolysaccharide / Wzx/Wzy pathway / polysaccharide polymerase / polysaccharide co-polymerase |