EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Direct observation of backtracking by influenza A and B polymerases upon consecutive incorporation of the nucleoside analog T1106.
ジャーナル・号・ページ	Cell Rep, Vol. 42, Issue 1, Page 111901, Year 2023
掲載日	2023年1月31日
著者	Tomas Kouba / Anna Dubankova / Petra Drncova / Elisa Donati / Pietro Vidossich / Valentina Speranzini / Alex Pflug / Johanna Huchting / Chris Meier / Marco De Vivo / Stephen Cusack /
PubMed 要旨	The antiviral pseudo-base T705 and its de-fluoro analog T1106 mimic adenine or guanine and can be competitively incorporated into nascent RNA by viral RNA-dependent RNA polymerases. Although ...The antiviral pseudo-base T705 and its de-fluoro analog T1106 mimic adenine or guanine and can be competitively incorporated into nascent RNA by viral RNA-dependent RNA polymerases. Although dispersed, single pseudo-base incorporation is mutagenic, consecutive incorporation causes polymerase stalling and chain termination. Using a template encoding single and then consecutive T1106 incorporation four nucleotides later, we obtained a cryogenic electron microscopy structure of stalled influenza A/H7N9 polymerase. This shows that the entire product-template duplex backtracks by 5 nt, bringing the singly incorporated T1106 to the +1 position, where it forms an unexpected T1106:U wobble base pair. Similar structures show that influenza B polymerase also backtracks after consecutive T1106 incorporation, regardless of whether prior single incorporation has occurred. These results give insight into the unusual mechanism of chain termination by pyrazinecarboxamide base analogs. Consecutive incorporation destabilizes the proximal end of the product-template duplex, promoting irreversible backtracking to a more energetically favorable overall configuration.
リンク	Cell Rep / PubMed:36596301
手法	EM (単粒子)
解像度	2.34 - 3.12 Å
構造データ	14144 7qtl EMDB-14144, PDB-7qtl: Influenza A/H7N9 polymerase elongation complex 手法: EM (単粒子) / 解像度: 2.48 Å 14222 7r0e EMDB-14222, PDB-7r0e: Early transcription elongation state of influenza A/H7N9 polymerase backtracked due to double incoproation of nucleotide analogue T1106 and with singly incoporated T1106 at the +1 position 手法: EM (単粒子) / 解像度: 2.51 Å 14240 7r1f EMDB-14240, PDB-7r1f: Early transcription elongation state of influenza B polymerase backtracked due to double incoproation of nucleotide analogue T1106 手法: EM (単粒子) / 解像度: 2.58 Å 14857 7zpl EMDB-14857, PDB-7zpl: Symmetric dimer of influenza A/H7N9 polymerase bound to 5' vRNA hook 手法: EM (単粒子) / 解像度: 3.12 Å 14858 7zpm EMDB-14858, PDB-7zpm: Influenza A/H7N9 polymerase apo-protein dimer complex 手法: EM (単粒子) / 解像度: 2.81 Å 15984 8bdr EMDB-15984, PDB-8bdr: Early transcription elongation state of influenza B/Mem polymerase backtracked due to double incoproation of nucleotide analogue T1106 and with singly incoporated T1106 at the U +1 position 手法: EM (単粒子) / 解像度: 2.7 Å 15996 8be0 EMDB-15996, PDB-8be0: Early transcription elongation state of influenza B/Mem polymerase backtracked due to double incoproation of nucleotide analogue T1106 and with singly incoporated T1106 at the C +1 position 手法: EM (単粒子) / 解像度: 2.34 Å 16006 8bek EMDB-16006, PDB-8bek: Early transcription elongation state of influenza A/H7N9 backtracked polymerase with singly incoporated T1106 at the U +1 position 手法: EM (単粒子) / 解像度: 2.86 Å 16013 8bf5 EMDB-16013, PDB-8bf5: Early transcription elongation state of influenza A/H7N9 polymerase stalled with incoming GTP analogue 手法: EM (単粒子) / 解像度: 2.96 Å
化合物	ChemComp-2TM: 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine / 5′-シチジルオキシホスホニルメチルホスホニルオキシホスホン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GTA: P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE / 7-メチルグアノシン-7-イウム5′-三りん酸O^γ-(5′-アデノシル) ChemComp-HOH: WATER ChemComp-G2P: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMPCPP / GMP-CPP, エネルギー貯蔵分子類似体*YM
由来	influenza a virus (a/zhejiang/dtid-zju01/2013(h7n9)) (A型インフルエンザウイルス) influenza a virus (A型インフルエンザウイルス) influenza b virus (b/memphis/13/2003) (B型インフルエンザウイルス) influenza b virus (B型インフルエンザウイルス) influenza b virus (b/acre/117700/2012) (B型インフルエンザウイルス) influenza b virus (b/kaliningrad/rii06/2012) (B型インフルエンザウイルス)
キーワード	VIRAL PROTEIN / Influenza / viral RNA-dependent RNA polymerase; cap-dependent transcription / viral RNA-dependent RNA polymerase; antiviral drug; nucleoside analogue; T705 (favipiravir); T1106; cap-dependent transcription; backtracking; / H7N9 / viral RNA-dependent RNA polymerase / viral RNA-dependent RNA polymerase; / nucleoside analogue