EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of Streptomyces transcription activation by zinc uptake regulator.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 50, Issue 14, Page 8363-8376, Year 2022
掲載日	2022年8月12日
著者	Xu Yang / Yiqun Wang / Guiyang Liu / Zixin Deng / Shuangjun Lin / Jianting Zheng /
PubMed 要旨	Streptomyces coelicolor (Sc) is a model organism of actinobacteria to study morphological differentiation and production of bioactive metabolites. Sc zinc uptake regulator (Zur) affects both ...Streptomyces coelicolor (Sc) is a model organism of actinobacteria to study morphological differentiation and production of bioactive metabolites. Sc zinc uptake regulator (Zur) affects both processes by controlling zinc homeostasis. It activates transcription by binding to palindromic Zur-box sequences upstream of -35 elements. Here we deciphered the molecular mechanism by which ScZur interacts with promoter DNA and Sc RNA polymerase (RNAP) by cryo-EM structures and biochemical assays. The ScZur-DNA structures reveal a sequential and cooperative binding of three ScZur dimers surrounding a Zur-box spaced 8 nt upstream from a -35 element. The ScRNAPσHrdB-Zur-DNA structures define protein-protein and protein-DNA interactions involved in the principal housekeeping σHrdB-dependent transcription initiation from a noncanonical promoter with a -10 element lacking the critical adenine residue at position -11 and a TTGCCC -35 element deviating from the canonical TTGACA motif. ScZur interacts with the C-terminal domain of ScRNAP α subunit (αCTD) in a complex structure trapped in an active conformation. Key ScZur-αCTD interfacial residues accounting for ScZur-dependent transcription activation were confirmed by mutational studies. Together, our structural and biochemical results provide a comprehensive model for transcription activation of Zur family regulators.
リンク	Nucleic Acids Res / PubMed:35871291 / PubMed Central
手法	EM (単粒子)
解像度	3.4 - 10.8 Å
構造データ	32048 7vo0 EMDB-32048, PDB-7vo0: Streptomyces coelicolor zinc uptake regulator complexed with zinc and DNA (trimer of dimers) 手法: EM (単粒子) / 解像度: 3.4 Å 32049 7vo9 EMDB-32049, PDB-7vo9: Streptomyces coelicolor zinc uptake regulator complexed with zinc and DNA (dimer of dimers) 手法: EM (単粒子) / 解像度: 3.8 Å 32063 7vpd EMDB-32063, PDB-7vpd: Cryo-EM structure of Streptomyces coelicolor RNAP-promoter open complex with one Zur dimers 手法: EM (単粒子) / 解像度: 3.77 Å 32077 7vpz EMDB-32077, PDB-7vpz: Cryo-EM structure of Streptomyces coelicolor transcription initial complex with one Zur dimer 手法: EM (単粒子) / 解像度: 4.14 Å 33031 7x74 EMDB-33031: Cryo-EM structure of Streptomyces coelicolor transcription initial complex with two Zur dimers PDB-7x74: Cryo-EM structure of Streptomyces coelicolor transcription initial complex with two Zur dimers. 手法: EM (単粒子) / 解像度: 3.7 Å 33032 7x75 EMDB-33032, PDB-7x75: Cryo-EM structure of Streptomyces coelicolor RNAP-promoter open complex with three Zur dimers 手法: EM (単粒子) / 解像度: 3.45 Å 33033 7x76 EMDB-33033, PDB-7x76: Cryo-EM structure of Streptomyces coelicolor RNAP-promoter open complex with two Zur dimers 手法: EM (単粒子) / 解像度: 3.67 Å EMDB-33034: Focused map on the aCTD-Zur region of the Streptomyces coelicolor RNAP-promoter open complex with one Zur dimer 手法: EM (単粒子) / 解像度: 10.5 Å EMDB-33035: Focused map on the Zur region of the Streptomyces coelicolor RNAP-promoter open complex with two Zur dimers 手法: EM (単粒子) / 解像度: 9.4 Å EMDB-33036: Local refinement map of Zur regions in Streptomyces coelicolor RNAP-promoter open complex with three Zur dimers 手法: EM (単粒子) / 解像度: 5.37 Å EMDB-33037: Focused map on the Zur region of the Streptomyces coelicolor transcription initial complex with one Zur dimer 手法: EM (単粒子) / 解像度: 10.8 Å EMDB-33038: Focused map on the Zur region of the Streptomyces coelicolor transcription initial complex with two Zur dimers 手法: EM (単粒子) / 解像度: 7.9 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	streptomyces coelicolor a3(2) (バクテリア) streptomyces coelicolor (strain atcc baa-471 / a3(2) / m145) (バクテリア)
キーワード	DNA BINDING PROTEIN / GENE REGULATION / rna polymerase / zinc uptake regulator / Streptomyces / Open complex