EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of adhesion GPCR GPR110 activation by stalk peptide and G-proteins coupling.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 5513, Year 2022
掲載日	2022年9月20日
著者	Xinyan Zhu / Yu Qian / Xiaowan Li / Zhenmei Xu / Ruixue Xia / Na Wang / Jiale Liang / Han Yin / Anqi Zhang / Changyou Guo / Guangfu Wang / Yuanzheng He /
PubMed 要旨	Adhesion G protein-coupled receptors (aGPCRs) are keys of many physiological events and attractive targets for various diseases. aGPCRs are also known to be capable of self-activation via an ...Adhesion G protein-coupled receptors (aGPCRs) are keys of many physiological events and attractive targets for various diseases. aGPCRs are also known to be capable of self-activation via an autoproteolysis process that removes the inhibitory GAIN domain on the extracellular side of receptor and releases a stalk peptide to bind and activate the transmembrane side of receptor. However, the detailed mechanism of aGPCR activation remains elusive. Here, we report the cryo-electron microscopy structures of GPR110 (ADGRF1), a member of aGPCR, in complex with G, G, G, G and G The structures reveal distinctive ligand engaging model and activation conformations of GPR110. The structures also unveil the rarely explored GPCR/G and GPCR/G engagements. A comparison of G, G, G, G and G engagements with GPR110 reveals details of G-protein engagement, including a dividing point at the far end of the alpha helix 5 (αH5) of Gα subunit that separates G/G engagements from G/G/G engagements. This is also where G/G bind the receptor through both hydrophobic and polar interaction, while G/G/G engage receptor mainly through hydrophobic interaction. We further provide physiological evidence of GPR110 activation via stalk peptide. Taken together, our study fills the missing information of GPCR/G-protein engagement and provides a framework for understanding aGPCR activation and GPR110 signaling.
リンク	Nat Commun / PubMed:36127364 / PubMed Central
手法	EM (単粒子)
解像度	2.83 - 3.09 Å
構造データ	32881 7wxu EMDB-32881: Protein 110 and Q complex PDB-7wxu: GPR110/Gq complex 手法: EM (単粒子) / 解像度: 2.85 Å 32882 7wxw EMDB-32882: Protein 110 and S complex PDB-7wxw: GPR110/Gs complex 手法: EM (単粒子) / 解像度: 2.84 Å 32883 7wy0 EMDB-32883: Protein 110 and 13 complex PDB-7wy0: GPR110/G13 complex 手法: EM (単粒子) / 解像度: 2.83 Å 32905 7wz7 EMDB-32905: Protein 110 and 12 complex PDB-7wz7: GPR110/G12 complex 手法: EM (単粒子) / 解像度: 2.83 Å 32972 7x2v EMDB-32972: Protein 110 and i complex PDB-7x2v: GPR110/Gi complex 手法: EM (単粒子) / 解像度: 3.09 Å
由来	homo sapiens (ヒト) lama glama (ラマ)
キーワード	MEMBRANE PROTEIN / GPCR