EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of dynamic P5CS filaments.
ジャーナル・号・ページ	Elife, Vol. 11, Year 2022
掲載日	2022年3月14日
著者	Jiale Zhong / Chen-Jun Guo / Xian Zhou / Chia-Chun Chang / Boqi Yin / Tianyi Zhang / Huan-Huan Hu / Guang-Ming Lu / Ji-Long Liu /
PubMed 要旨	The bifunctional enzyme Δ-pyrroline-5-carboxylate synthase (P5CS) is vital to the synthesis of proline and ornithine, playing an essential role in human health and agriculture. Pathogenic mutations ...The bifunctional enzyme Δ-pyrroline-5-carboxylate synthase (P5CS) is vital to the synthesis of proline and ornithine, playing an essential role in human health and agriculture. Pathogenic mutations in the P5CS gene (ALDH18A1) lead to neurocutaneous syndrome and skin relaxation connective tissue disease in humans, and P5CS deficiency seriously damages the ability to resist adversity in plants. We have recently found that P5CS forms cytoophidia in vivo and filaments in vitro. However, it is difficult to appreciate the function of P5CS filamentation without precise structures. Using cryo-electron microscopy, here we solve the structures of full-length P5CS in three states at resolution from 3.1 to 4.3 Å. We observe distinct ligand-binding states and conformational changes for the GK and GPR domains, respectively. Divergent helical filaments are assembled by P5CS tetramers and stabilized by multiple interfaces. Point mutations disturbing those interfaces prevent P5CS filamentation and greatly reduce the enzymatic activity. Our findings reveal that filamentation is crucial for the coordination between the GK and GPR domains, providing a structural basis for the catalytic function of P5CS filaments.
リンク	Elife / PubMed:35286254 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 4.3 Å
構造データ	31466 7f5t EMDB-31466: Structural basis of assembly and activity of P5CS filament PDB-7f5t: Drosophila P5CS filament with glutamate 手法: EM (単粒子) / 解像度: 4.1 Å 31467 7f5u EMDB-31467, PDB-7f5u: Drosophila P5CS filament with glutamate and ATPgammaS 手法: EM (単粒子) / 解像度: 4.1 Å 31468 7f5v EMDB-31468, PDB-7f5v: Drosophila P5CS filament with glutamate, ATP, and NADPH 手法: EM (単粒子) / 解像度: 3.6 Å 31469 7f5x EMDB-31469, PDB-7f5x: GK domain of Drosophila P5CS filament with glutamate 手法: EM (単粒子) / 解像度: 3.5 Å 32875 7wx3 EMDB-32875, PDB-7wx3: GK domain of Drosophila P5CS filament with glutamate, ATP, and NADPH 手法: EM (単粒子) / 解像度: 3.1 Å 32876 7wx4 EMDB-32876, PDB-7wx4: GK domain of Drosophila P5CS filament with glutamate and ATPgammaS 手法: EM (単粒子) / 解像度: 3.4 Å 32877 7wxf EMDB-32877, PDB-7wxf: GPR domain of Drosophila P5CS filament with glutamate 手法: EM (単粒子) / 解像度: 3.6 Å 32878 7wxg EMDB-32878, PDB-7wxg: GPR domain closed form of Drosophila P5CS filament with glutamate, ATP, and NADPH 手法: EM (単粒子) / 解像度: 4.2 Å 32879 7wxh EMDB-32879, PDB-7wxh: GPR domain open form of Drosophila P5CS filament with glutamate, ATP, and NADPH 手法: EM (単粒子) / 解像度: 4.3 Å 32880 7wxi EMDB-32880, PDB-7wxi: GPR domain of Drosophila P5CS filament with glutamate and ATPgammaS 手法: EM (単粒子) / 解像度: 4.2 Å
化合物	ChemComp-GLU: GLUTAMIC ACID / グルタミン酸 ChemComp-GGL: GAMMA-L-GLUTAMIC ACID / グルタミン酸 ChemComp-RGP: GAMMA-GLUTAMYL PHOSPHATE ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-NAP*: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
由来	drosophila melanogaster (キイロショウジョウバエ)
キーワード	TRANSFERASE / glutamate-bound / filament / ALDH18A1 / Delta-1-pyrroline-5-carboxylate synthase / BIOSYNTHETIC PROTEIN