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-Structure paper
タイトル | In vitro structural maturation of an early stage pre-40S particle coupled with U3 snoRNA release and central pseudoknot formation. |
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ジャーナル・号・ページ | Nucleic Acids Res, Vol. 50, Issue 20, Page 11916-11923, Year 2022 |
掲載日 | 2022年11月11日 |
著者 | Jingdong Cheng / Giuseppe La Venuta / Benjamin Lau / Otto Berninghausen / Roland Beckmann / Ed Hurt / |
PubMed 要旨 | The transition of the 90S to the pre-40S pre-ribosome is a decisive step in eukaryotic small subunit biogenesis leading to a first pre-40S intermediate (state Dis-C or primordial pre-40S), where the ...The transition of the 90S to the pre-40S pre-ribosome is a decisive step in eukaryotic small subunit biogenesis leading to a first pre-40S intermediate (state Dis-C or primordial pre-40S), where the U3 snoRNA keeps the nascent 18S rRNA locally immature. We in vitro reconstitute the ATP-dependent U3 release from this particle, catalyzed by the helicase Dhr1, and follow this process by cryo-EM revealing two successive pre-40S intermediates, Dis-D and Dis-E. The latter has lost not only U3 but all residual 90S factors including the GTPase Bms1. In vitro remodeling likewise induced the formation of the central pseudoknot, a universally conserved tertiary RNA structure that comprises the core of the small subunit decoding center. Thus, we could structurally reveal a key tertiary RNA folding step that is essential to form the active 40S subunit. |
リンク | Nucleic Acids Res / PubMed:36263816 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.3 - 3.5 Å |
構造データ | EMDB-32790, PDB-7wtl: EMDB-32791, PDB-7wtm: |
化合物 | ChemComp-ZN: ChemComp-GTP: ChemComp-MG: |
由来 |
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キーワード | RIBOSOME / ribosome biogenesis / 40S ribosome |