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-Structure paper
タイトル | Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation. |
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ジャーナル・号・ページ | Science, Vol. 374, Issue 6571, Page 1152-1157, Year 2021 |
掲載日 | 2021年11月26日 |
著者 | Qian Wang / Yan Xue / Laixing Zhang / Zhenhui Zhong / Suhua Feng / Changshi Wang / Lifan Xiao / Zhenlin Yang / C Jake Harris / Zhe Wu / Jixian Zhai / Maojun Yang / Sisi Li / Steven E Jacobsen / Jiamu Du / |
PubMed 要旨 | In eukaryotes, small RNAs (sRNAs) play critical roles in multiple biological processes. Dicer endonucleases are a central part of sRNA biogenesis. In plants, DICER-LIKE PROTEIN 3 (DCL3) produces 24- ...In eukaryotes, small RNAs (sRNAs) play critical roles in multiple biological processes. Dicer endonucleases are a central part of sRNA biogenesis. In plants, DICER-LIKE PROTEIN 3 (DCL3) produces 24-nucleotide (nt) small interfering RNAs (siRNAs) that determine the specificity of the RNA-directed DNA methylation pathway. Here, we determined the structure of a DCL3–pre-siRNA complex in an active dicing-competent state. The 5′-phosphorylated A1 of the guide strand and the 1-nt 3′ overhang of the complementary strand are specifically recognized by a positively charged pocket and an aromatic cap, respectively. The 24-nt siRNA length dependence relies on the separation between the 5′-phosphorylated end of the guide RNA and dual cleavage sites formed by the paired ribonuclease III domains. These structural studies, complemented by functional data, provide insight into the dicing principle for Dicers in general. |
リンク | Science / PubMed:34648373 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.1 - 3.73 Å |
構造データ | EMDB-31963, PDB-7vg2: EMDB-31964, PDB-7vg3: |
化合物 | ChemComp-CA: ChemComp-ZN: |
由来 |
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キーワード | RNA BINDING PROTEIN/RNA / DCL3 / RNA / RdDM / Dicer / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex |