EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	rRNA methylation by Spb1 regulates the GTPase activity of Nog2 during 60S ribosomal subunit assembly.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 1207, Year 2023
掲載日	2023年3月2日
著者	Kamil Sekulski / Victor Emmanuel Cruz / Christine S Weirich / Jan P Erzberger /
PubMed 要旨	Biogenesis of the large ribosomal (60S) subunit involves the assembly of three rRNAs and 46 proteins, a process requiring approximately 70 ribosome biogenesis factors (RBFs) that bind and release the ...Biogenesis of the large ribosomal (60S) subunit involves the assembly of three rRNAs and 46 proteins, a process requiring approximately 70 ribosome biogenesis factors (RBFs) that bind and release the pre-60S at specific steps along the assembly pathway. The methyltransferase Spb1 and the K-loop GTPase Nog2 are essential RBFs that engage the rRNA A-loop during sequential steps in 60S maturation. Spb1 methylates the A-loop nucleotide G2922 and a catalytically deficient mutant strain (spb1) has a severe 60S biogenesis defect. However, the assembly function of this modification is currently unknown. Here, we present cryo-EM reconstructions that reveal that unmethylated G2922 leads to the premature activation of Nog2 GTPase activity and capture a Nog2-GDP-AlF transition state structure that implicates the direct involvement of unmodified G2922 in Nog2 GTPase activation. Genetic suppressors and in vivo imaging indicate that premature GTP hydrolysis prevents the efficient binding of Nog2 to early nucleoplasmic 60S intermediates. We propose that G2922 methylation levels regulate Nog2 recruitment to the pre-60S near the nucleolar/nucleoplasmic phase boundary, forming a kinetic checkpoint to regulate 60S production. Our approach and findings provide a template to study the GTPase cycles and regulatory factor interactions of the other K-loop GTPases involved in ribosome assembly.
リンク	Nat Commun / PubMed:36864048 / PubMed Central
手法	EM (単粒子)
解像度	2.34 - 2.9 Å
構造データ	26651 7uoo EMDB-26651, PDB-7uoo: Nucleoplasmic pre-60S intermediate of the Nog2 containing pre-rotation state 手法: EM (単粒子) / 解像度: 2.34 Å 26686 7uqb EMDB-26686, PDB-7uqb: Nucleoplasmic pre-60S intermediate of the Nog2 containing pre-rotation state from a SPB1-D52A strain with AlF4 手法: EM (単粒子) / 解像度: 2.43 Å EMDB-26689: Locally refined 5S rRNP map from the nucleoplasmic pre-60S intermediate of the Nog2 containing pre-rotation state 手法: EM (単粒子) / 解像度: 2.63 Å 26703 7uqz EMDB-26703, PDB-7uqz: Nucleoplasmic pre-60S intermediate of the Nog2 containing pre-rotation state from a SPB1 D52A strain 手法: EM (単粒子) / 解像度: 2.44 Å 26799 7uui EMDB-26799, PDB-7uui: Nucleoplasmic pre-60S intermediate of the Nog2 containing post-rotation state from a SPB1 D52A strain 手法: EM (単粒子) / 解像度: 2.9 Å 26941 7v08 EMDB-26941, PDB-7v08: Nucleoplasmic pre-60S intermediate of the Nog2 containing pre-rotation state from a Spb1 D52A suppressor 3 strain 手法: EM (単粒子) / 解像度: 2.36 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-B3P: 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / 2,2′-(トリメチレンビスイミノ)ビス[2-(ヒドロキシメチル)プロパン-1,3-ジオ-ル] / pH緩衝剤YM ChemComp-ZN: Unknown entry ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-K: Unknown entry / カリウムカチオン ChemComp-HOH: WATER ChemComp-ALF*: TETRAFLUOROALUMINATE ION / テトラフルオロアルミナ-ト
由来	saccharomyces cerevisiae by4741 (パン酵母)
キーワード	RIBOSOME / ribosome biogenesis / K-loop GTPase / GTPase / GTPase transition state / GDP aluminum fluoride / 5S rRNP