EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 29, Issue 12, Page 1228-1238, Year 2022
掲載日	2022年12月8日
著者	Victor Emmanuel Cruz / Kamil Sekulski / Nagesh Peddada / Carolin Sailer / Sahana Balasubramanian / Christine S Weirich / Florian Stengel / Jan P Erzberger /
PubMed 要旨	DEAD-box ATPases are ubiquitous enzymes essential in all aspects of RNA biology. However, the limited in vitro catalytic activities described for these enzymes are at odds with their complex cellular ...DEAD-box ATPases are ubiquitous enzymes essential in all aspects of RNA biology. However, the limited in vitro catalytic activities described for these enzymes are at odds with their complex cellular roles, most notably in driving large-scale RNA remodeling steps during the assembly of ribonucleoproteins (RNPs). We describe cryo-EM structures of 60S ribosomal biogenesis intermediates that reveal how context-specific RNA unwinding by the DEAD-box ATPase Spb4 results in extensive, sequence-specific remodeling of rRNA secondary structure. Multiple cis and trans interactions stabilize Spb4 in a post-catalytic, high-energy intermediate that drives the organization of the three-way junction at the base of rRNA domain IV. This mechanism explains how limited strand separation by DEAD-box ATPases is leveraged to provide non-equilibrium directionality and ensure efficient and accurate RNP assembly.
リンク	Nat Struct Mol Biol / PubMed:36482249 / PubMed Central
手法	EM (単粒子)
解像度	2.76 - 3.71 Å
構造データ	24269 7nac EMDB-24269: State E2 nucleolar 60S ribosomal biogenesis intermediate - Overall map PDB-7nac: State E2 nucleolar 60S ribosomal biogenesis intermediate - Composite model 手法: EM (単粒子) / 解像度: 3.04 Å 24270 7nad EMDB-24270, PDB-7nad: State E2 nucleolar 60S ribosomal biogenesis intermediate - Spb4 local refinement model 手法: EM (単粒子) / 解像度: 3.04 Å 24271 7naf EMDB-24271: State E2 nucleolar 60S ribosomal biogenesis intermediate - Spb1-MTD locally refined map PDB-7naf: State E2 nucleolar 60S ribosomal biogenesis intermediate - Spb1-MTD local model 手法: EM (単粒子) / 解像度: 3.13 Å 24280 7r6k EMDB-24280: State E2 nucleolar 60S ribosomal intermediate - Local Map for Noc2/Noc3 region PDB-7r6k: State E2 nucleolar 60S ribosomal intermediate - Model for Noc2/Noc3 region 手法: EM (単粒子) / 解像度: 3.17 Å 24286 7r6q EMDB-24286: State E2 nucleolar 60S ribosome biogenesis intermediate - Foot region map PDB-7r6q: State E2 nucleolar 60S ribosome biogenesis intermediate - Foot region model 手法: EM (単粒子) / 解像度: 2.98 Å 24290 7r72 EMDB-24290: State E1 nucleolar 60S ribosome biogenesis intermediate - Spb4 locally refined map PDB-7r72: State E1 nucleolar 60S ribosome biogenesis intermediate - Spb4 local model 手法: EM (単粒子) / 解像度: 3.07 Å 24296 7r7a EMDB-24296, PDB-7r7a: State E1 nucleolar 60S ribosome biogenesis intermediate - Composite model 手法: EM (単粒子) / 解像度: 3.04 Å 24297 7r7c EMDB-24297: State E2 nucleolar 60S ribosomal biogenesis intermediate - L1 stalk local map PDB-7r7c: State E2 nucleolar 60S ribosomal biogenesis intermediate - L1 stalk local model 手法: EM (単粒子) / 解像度: 3.71 Å 26259 7u0h EMDB-26259: State NE1 nucleolar 60S ribosome biogenesis intermediate - Overall map PDB-7u0h: State NE1 nucleolar 60S ribosome biogenesis intermediate - Overall model 手法: EM (単粒子) / 解像度: 2.76 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE / S-アデノシルホモシステイン ChemComp-HOH: WATER ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae by4741 (パン酵母)
キーワード	RIBOSOME / ribosome biogenesis / DEAD-box ATPases / methyltransferase / nucleolus / methyltransferases / DEAD-box ATPase