EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of a monomeric photosystem II core complex from a cyanobacterium acclimated to far-red light reveals the functions of chlorophylls d and f.
ジャーナル・号・ページ	J Biol Chem, Vol. 298, Issue 1, Page 101424, Year 2022
掲載日	2021年11月19日
著者	Christopher J Gisriel / Gaozhong Shen / Ming-Yang Ho / Vasily Kurashov / David A Flesher / Jimin Wang / William H Armstrong / John H Golbeck / Marilyn R Gunner / David J Vinyard / Richard J Debus / Gary W Brudvig / Donald A Bryant /
PubMed 要旨	Far-red light (FRL) photoacclimation in cyanobacteria provides a selective growth advantage for some terrestrial cyanobacteria by expanding the range of photosynthetically active radiation to include ...Far-red light (FRL) photoacclimation in cyanobacteria provides a selective growth advantage for some terrestrial cyanobacteria by expanding the range of photosynthetically active radiation to include far-red/near-infrared light (700-800 nm). During this photoacclimation process, photosystem II (PSII), the water:plastoquinone photooxidoreductase involved in oxygenic photosynthesis, is modified. The resulting FRL-PSII is comprised of FRL-specific core subunits and binds chlorophyll (Chl) d and Chl f molecules in place of several of the Chl a molecules found when cells are grown in visible light. These new Chls effectively lower the energy canonically thought to define the "red limit" for light required to drive photochemical catalysis of water oxidation. Changes to the architecture of FRL-PSII were previously unknown, and the positions of Chl d and Chl f molecules had only been proposed from indirect evidence. Here, we describe the 2.25 Å resolution cryo-EM structure of a monomeric FRL-PSII core complex from Synechococcus sp. PCC 7335 cells that were acclimated to FRL. We identify one Chl d molecule in the Chl position of the electron transfer chain and four Chl f molecules in the core antenna. We also make observations that enhance our understanding of PSII biogenesis, especially on the acceptor side of the complex where a bicarbonate molecule is replaced by a glutamate side chain in the absence of the assembly factor Psb28. In conclusion, these results provide a structural basis for the lower energy limit required to drive water oxidation, which is the gateway for most solar energy utilization on earth.
リンク	J Biol Chem / PubMed:34801554 / PubMed Central
手法	EM (単粒子)
解像度	2.25 Å
構造データ	24943 7sa3 EMDB-24943, PDB-7sa3: Structure of a monomeric photosystem II core complex from a cyanobacterium acclimated to far-red light 手法: EM (単粒子) / 解像度: 2.25 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-CL: Unknown entry / クロリド / 塩化物 ChemComp-CLA: CHLOROPHYLL A / クロロフィルa ChemComp-CL7: CHLOROPHYLL D / Chlorophyll d ChemComp-PHO: PHEOPHYTIN A / 13²α-(メトキシカルボニル)-3¹,3²-ジデヒ(以下略) / フェオフィチン ChemComp-BCR: BETA-CAROTENE / β-カロチン / Β-カロテン ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM 化合物画像なし ChemComp-F6C: Chlorophyll F / Chlorophyll f ChemComp-DGD: DIGALACTOSYL DIACYL GLYCEROL (DGDG) ChemComp-LMG: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE ChemComp-FE2: Unknown entry ChemComp-PL9: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE / プラストキノン ChemComp-LHG: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / ジパルミトイルホスファチジルグリセロ-ル / リン脂質YM / Phosphatidylglycerol ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE / Heme B ChemComp-HOH: WATER / 水
由来	synechococcus sp. pcc 7335 (バクテリア)
キーワード	PHOTOSYNTHESIS (光合成) / Photosystem II (光化学系II) / far-red light photoacclimation / chlorophyll f / chlorophyll d / bicarbonate (炭酸水素塩) / photoactivation / cyanobacteria (藍藻)