EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural mechanisms of assembly, permeation, gating, and pharmacology of native human rod CNG channel.
ジャーナル・号・ページ	Neuron, Vol. 110, Issue 1, Page 86-95.e5, Year 2022
掲載日	2022年1月5日
著者	Jing Xue / Yan Han / Weizhong Zeng / Youxing Jiang /
PubMed 要旨	Mammalian cyclic nucleotide-gated (CNG) channels are nonselective cation channels activated by cGMP or cAMP and play essential roles in the signal transduction of the visual and olfactory sensory ...Mammalian cyclic nucleotide-gated (CNG) channels are nonselective cation channels activated by cGMP or cAMP and play essential roles in the signal transduction of the visual and olfactory sensory systems. CNGA1, the principal component of the CNG channel from rod photoreceptors, can by itself form a functional homotetrameric channel and has been used as the model system in the majority of rod CNG studies. However, the native rod CNG functions as a heterotetramer consisting of three A1 and one B1 subunits and exhibits different functional properties than the CNGA1 homomer. Here we present the functional analysis of human rod CNGA1/B1 heterotetramer and its cryo-EM structures in apo, cGMP-bound, cAMP-bound, and L-cis-Diltiazem-blocked states. These structures, with resolution ranging from 2.6 to 3.3 Å, elucidate the structural mechanisms underlying the 3:1 subunit stoichiometry, the asymmetrical gating upon cGMP activation, and the unique pharmacological property of the native rod CNG channel.
リンク	Neuron / PubMed:34699778 / PubMed Central
手法	EM (単粒子)
解像度	2.61 - 3.31 Å
構造データ	24458 7rh9 EMDB-24458, PDB-7rh9: Cryo-EM structure of human rod CNGA1/B1 channel in apo state 手法: EM (単粒子) / 解像度: 2.61 Å 24460 7rhg EMDB-24460, PDB-7rhg: Cryo-EM structure of human rod CNGA1/B1 channel in cAMP-bound state 手法: EM (単粒子) / 解像度: 2.88 Å 24461 7rhh EMDB-24461, PDB-7rhh: Cryo-EM structure of human rod CNGA1/B1 channel in cGMP-bound openI state 手法: EM (単粒子) / 解像度: 3.31 Å 24462 7rhi EMDB-24462, PDB-7rhi: Cryo-EM structure of human rod CNGA1/B1 channel in cGMP-bound openII state 手法: EM (単粒子) / 解像度: 3.31 Å 24463 7rhj EMDB-24463, PDB-7rhj: Cryo-EM structure of human rod CNGA1/B1 channel in L-cis-Diltiazem-blocked open state 手法: EM (単粒子) / 解像度: 2.88 Å 24464 7rhk EMDB-24464, PDB-7rhk: Cryo-EM structure of human rod CNGA1/B1 channel in L-cis-Diltiazem-trapped closed state 手法: EM (単粒子) / 解像度: 3.27 Å 24465 7rhl EMDB-24465, PDB-7rhl: Cryo-EM structure of human rod Apo CNGA1/B1 channel with CLZ coiled coil 手法: EM (単粒子) / 解像度: 3.03 Å
化合物	ChemComp-CMP: ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE / cAMP ChemComp-PCG: CYCLIC GUANOSINE MONOPHOSPHATE / cGMP ChemComp-5H0: (2R,3R)-5-[2-(dimethylamino)ethyl]-2-(4-methoxyphenyl)-4-oxo-2,3,4,5-tetrahydro-1,5-benzothiazepin-3-yl acetate / l-cis-ジルチアゼム
由来	homo sapiens (ヒト)
キーワード	TRANSPORT PROTEIN / ion channel