EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Evolution of increased complexity and specificity at the dawn of form I Rubiscos.
ジャーナル・号・ページ	Science, Vol. 378, Issue 6616, Page 155-160, Year 2022
掲載日	2022年10月14日
著者	Luca Schulz / Zhijun Guo / Jan Zarzycki / Wieland Steinchen / Jan M Schuller / Thomas Heimerl / Simone Prinz / Oliver Mueller-Cajar / Tobias J Erb / Georg K A Hochberg /
PubMed 要旨	The evolution of ribulose-1,5-bisphosphate carboxylase/oxygenases (Rubiscos) that discriminate strongly between their substrate carbon dioxide and the undesired side substrate dioxygen was an ...The evolution of ribulose-1,5-bisphosphate carboxylase/oxygenases (Rubiscos) that discriminate strongly between their substrate carbon dioxide and the undesired side substrate dioxygen was an important event for photosynthetic organisms adapting to an oxygenated environment. We use ancestral sequence reconstruction to recapitulate this event. We show that Rubisco increased its specificity and carboxylation efficiency through the gain of an accessory subunit before atmospheric oxygen was present. Using structural and biochemical approaches, we retrace how this subunit was gained and became essential. Our work illuminates the emergence of an adaptation to rising ambient oxygen levels, provides a template for investigating the function of interactions that have remained elusive because of their essentiality, and sheds light on the determinants of specificity in Rubisco.
リンク	Science / PubMed:36227987
手法	EM (単粒子) / X線回折
解像度	1.8 - 3.0 Å
構造データ	14178 7qvi EMDB-14178, PDB-7qvi: Fiber-forming RubisCO derived from ancestral sequence reconstruction and rational engineering 手法: EM (単粒子) / 解像度: 3.0 Å PDB-7qsv: L8-complex forming RubisCO derived from ancestral sequence reconstruction of the last common ancestor of Form I'' and Form I RubisCOs 手法: X-RAY DIFFRACTION / 解像度: 2.1 Å PDB-7qsw: L8S8-complex forming RubisCO derived from ancestral sequence reconstruction of the last common ancestor of SSU-bearing Form I RubisCOs 手法: X-RAY DIFFRACTION / 解像度: 1.8 Å PDB-7qsx: Non-obligately L8S8-complex forming RubisCO derived from ancestral sequence reconstruction and rational engineering in L8 complex 手法: X-RAY DIFFRACTION / 解像度: 2.7 Å PDB-7qsy: Non-obligately L8S8-complex forming RubisCO derived from ancestral sequence reconstruction and rational engineering in L8S8 complex 手法: X-RAY DIFFRACTION / 解像度: 2.1 Å PDB-7qsz: Non-obligately L8S8-complex forming RubisCO derived from ancestral sequence reconstruction and rational engineering in L8 complex with substitution e170N 手法: X-RAY DIFFRACTION / 解像度: 2.25 Å PDB-7qt1: Non-obligately L8S8-complex forming RubisCO derived from ancestral sequence reconstruction and rational engineering in L8S8 complex with substitution e170N 手法: X-RAY DIFFRACTION / 解像度: 2.1 Å
化合物	ChemComp-CAP: 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE / 2-カルボキシ-D-アラビニト-ル1,5-ビスりん酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER
由来	synthetic construct (人工物)
キーワード	LYASE / Ribulose 1 / 5-bisphosphate carboxylase/oxydase / RubisCO