EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures define ubiquinone-10 binding to mitochondrial complex I and conformational transitions accompanying Q-site occupancy.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 2758, Year 2022
掲載日	2022年5月19日
著者	Injae Chung / John J Wright / Hannah R Bridges / Bozhidar S Ivanov / Olivier Biner / Caroline S Pereira / Guilherme M Arantes / Judy Hirst /
PubMed 要旨	Mitochondrial complex I is a central metabolic enzyme that uses the reducing potential of NADH to reduce ubiquinone-10 (Q) and drive four protons across the inner mitochondrial membrane, powering ...Mitochondrial complex I is a central metabolic enzyme that uses the reducing potential of NADH to reduce ubiquinone-10 (Q) and drive four protons across the inner mitochondrial membrane, powering oxidative phosphorylation. Although many complex I structures are now available, the mechanisms of Q reduction and energy transduction remain controversial. Here, we reconstitute mammalian complex I into phospholipid nanodiscs with exogenous Q. Using cryo-EM, we reveal a Q molecule occupying the full length of the Q-binding site in the 'active' (ready-to-go) resting state together with a matching substrate-free structure, and apply molecular dynamics simulations to propose how the charge states of key residues influence the Q binding pose. By comparing ligand-bound and ligand-free forms of the 'deactive' resting state (that require reactivating to catalyse), we begin to define how substrate binding restructures the deactive Q-binding site, providing insights into its physiological and mechanistic relevance.
リンク	Nat Commun / PubMed:35589726 / PubMed Central
手法	EM (単粒子)
解像度	2.3 - 3.02 Å
構造データ	14132 7qsk EMDB-14132, PDB-7qsk: Bovine complex I in lipid nanodisc, Active-Q10 手法: EM (単粒子) / 解像度: 2.84 Å 14133 7qsl EMDB-14133, PDB-7qsl: Bovine complex I in lipid nanodisc, Active-apo 手法: EM (単粒子) / 解像度: 2.76 Å 14134 7qsm EMDB-14134, PDB-7qsm: Bovine complex I in lipid nanodisc, Deactive-ligand (composite) 手法: EM (単粒子) / 解像度: 2.3 Å 14139 7qsn EMDB-14139, PDB-7qsn: Bovine complex I in lipid nanodisc, Deactive-apo 手法: EM (単粒子) / 解像度: 2.81 Å 14140 7qso EMDB-14140, PDB-7qso: Bovine complex I in lipid nanodisc, State 3 (Slack) 手法: EM (単粒子) / 解像度: 3.02 Å
化合物	ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-U10: UBIQUINONE-10 / 2-(3,7,11,15,19,23,27,31,35,39-デカメチルテトラコンタ-2,6,10,14,18,22,(以下略) ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-FMN: FLAVIN MONONUCLEOTIDE / FMN ChemComp-K: Unknown entry / カリウムカチオン ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH ChemComp-ZN: Unknown entry ChemComp-EHZ: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate ChemComp-CHD: CHOLIC ACID / コ-ル酸 ChemComp-MYR: MYRISTIC ACID / ミリスチン酸 ChemComp-HOH: WATER ChemComp-GOL: GLYCEROL / グリセロ-ル ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤*YM
由来	bos taurus (ウシ) cattle (ウシ)
キーワード	OXIDOREDUCTASE / Mitochondrial complex I / Respiratory complex I / NADH:ubiquinone oxidoreductase / Ubiquinone / Nanodisc / ELECTRON TRANSPORT