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-Structure paper
タイトル | Conformational transitions and ligand-binding to a muscle-type nicotinic acetylcholine receptor. |
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ジャーナル・号・ページ | Neuron, Vol. 110, Issue 8, Page 1358-11370.e5, Year 2022 |
掲載日 | 2022年4月20日 |
![]() | Eleftherios Zarkadas / Eva Pebay-Peyroula / Mackenzie John Thompson / Guy Schoehn / Tomasz Uchański / Jan Steyaert / Christophe Chipot / Francois Dehez / John Edward Baenziger / Hugues Nury / ![]() ![]() ![]() ![]() |
PubMed 要旨 | Fast synaptic communication requires receptors that respond to the presence of neurotransmitter by opening an ion channel across the post-synaptic membrane. The muscle-type nicotinic acetylcholine ...Fast synaptic communication requires receptors that respond to the presence of neurotransmitter by opening an ion channel across the post-synaptic membrane. The muscle-type nicotinic acetylcholine receptor from the electric fish, Torpedo, is the prototypic ligand-gated ion channel, yet the structural changes underlying channel activation remain undefined. Here we use cryo-EM to solve apo and agonist-bound structures of the Torpedo nicotinic receptor embedded in a lipid nanodisc. Using both a direct biochemical assay to define the conformational landscape and molecular dynamics simulations to assay flux through the pore, we correlate structures with functional states and elucidate the motions that lead to pore activation of a heteromeric nicotinic receptor. We highlight an underappreciated role for the complementary subunit in channel gating, establish the structural basis for the differential agonist affinities of α/δ versus α /γ sites, and explain why nicotine is less potent at muscle nicotinic receptors compared to neuronal ones. |
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手法 | EM (単粒子) |
解像度 | 2.5 - 3.23 Å |
構造データ | EMDB-14048, PDB-7qko: EMDB-14064, PDB-7ql5: EMDB-14065, PDB-7ql6: |
化合物 | ![]() ChemComp-POV: ![]() ChemComp-NCT: ![]() ChemComp-NAG: ![]() ChemComp-HOH: ![]() ChemComp-CCE: |
由来 |
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![]() | MEMBRANE PROTEIN / pentameric ligand-gated ion channel / nicotinic receptor |