EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanism of mitoribosomal small subunit biogenesis and preinitiation.
ジャーナル・号・ページ	Nature, Vol. 606, Issue 7914, Page 603-608, Year 2022
掲載日	2022年6月8日
著者	Yuzuru Itoh / Anas Khawaja / Ivan Laptev / Miriam Cipullo / Ilian Atanassov / Petr Sergiev / Joanna Rorbach / Alexey Amunts /
PubMed 要旨	Mitoribosomes are essential for the synthesis and maintenance of bioenergetic proteins. Here we use cryo-electron microscopy to determine a series of the small mitoribosomal subunit (SSU) ...Mitoribosomes are essential for the synthesis and maintenance of bioenergetic proteins. Here we use cryo-electron microscopy to determine a series of the small mitoribosomal subunit (SSU) intermediates in complex with auxiliary factors, revealing a sequential assembly mechanism. The methyltransferase TFB1M binds to partially unfolded rRNA h45 that is promoted by RBFA, while the mRNA channel is blocked. This enables binding of METTL15 that promotes further rRNA maturation and a large conformational change of RBFA. The new conformation allows initiation factor mtIF3 to already occupy the subunit interface during the assembly. Finally, the mitochondria-specific ribosomal protein mS37 (ref. ) outcompetes RBFA to complete the assembly with the SSU-mS37-mtIF3 complex that proceeds towards mtIF2 binding and translation initiation. Our results explain how the action of step-specific factors modulate the dynamic assembly of the SSU, and adaptation of a unique protein, mS37, links the assembly to initiation to establish the catalytic human mitoribosome.
リンク	Nature / PubMed:35676484 / PubMed Central
手法	EM (単粒子)
解像度	2.56 - 3.19 Å
構造データ	13551 7pnt EMDB-13551, PDB-7pnt: Assembly intermediate of mouse mitochondrial ribosome small subunit without mS37 in complex with RbfA and Tfb1m 手法: EM (単粒子) / 解像度: 3.19 Å 13552 7pnu EMDB-13552, PDB-7pnu: Assembly intermediate of mouse mitochondrial ribosome small subunit without mS37 in complex with RbfA inward conformation 手法: EM (単粒子) / 解像度: 3.06 Å 13553 7pnv EMDB-13553, PDB-7pnv: Assembly intermediate of mouse mitochondrial ribosome small subunit without mS37 in complex with RbfA and Mettl15 手法: EM (単粒子) / 解像度: 3.06 Å 13554 7pnw EMDB-13554, PDB-7pnw: Mouse mitochondrial ribosome small subunit lacking m5U modification 手法: EM (単粒子) / 解像度: 3.09 Å 13555 7pnx EMDB-13555, PDB-7pnx: Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and METTL15 conformation a 手法: EM (単粒子) / 解像度: 2.76 Å 13556 7pny EMDB-13556, PDB-7pny: Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and METTL15 conformation b 手法: EM (単粒子) / 解像度: 3.06 Å 13557 7pnz EMDB-13557, PDB-7pnz: Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and METTL15 conformation c 手法: EM (単粒子) / 解像度: 3.09 Å 13558 7po0 EMDB-13558, PDB-7po0: Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and IF3 手法: EM (単粒子) / 解像度: 2.9 Å 13559 7po1 EMDB-13559, PDB-7po1: Initiation complex of human mitochondrial ribosome small subunit with IF3 手法: EM (単粒子) / 解像度: 2.92 Å 13560 7po2 EMDB-13560, PDB-7po2: Initiation complex of human mitochondrial ribosome small subunit with IF2, fMet-tRNAMet and mRNA 手法: EM (単粒子) / 解像度: 3.09 Å 13561 7po3 EMDB-13561, PDB-7po3: Human mitochondrial ribosome small subunit 手法: EM (単粒子) / 解像度: 2.92 Å 13562 7po4 EMDB-13562, PDB-7po4: Assembly intermediate of human mitochondrial ribosome large subunit (largely unfolded rRNA with MALSU1, L0R8F8 and ACP) 手法: EM (単粒子) / 解像度: 2.56 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-ZN: Unknown entry ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER / 鉄・硫黄クラスター ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-HOH: WATER / 水 ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE / S-アデノシルホモシステイン / S-アデノシル-L-ホモシステイン ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM / ニコチンアミドアデニンジヌクレオチド ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-FME: N-FORMYLMETHIONINE / N-ホルミルメチオニン / N-ホルミルメチオニン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-8Q1: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate
由来	mus musculus (ハツカネズミ) homo sapiens (ヒト)
キーワード	RIBOSOME (リボソーム) / Assembly intermediate / maturation / biogenesis (自然発生説) / small subunit (サブユニット) / mitochondrion (ミトコンドリア) / Initiation factor 3 / translation (翻訳 (生物学))