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-Structure paper
タイトル | Mechanism of mitoribosomal small subunit biogenesis and preinitiation. |
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ジャーナル・号・ページ | Nature, Vol. 606, Issue 7914, Page 603-608, Year 2022 |
掲載日 | 2022年6月8日 |
著者 | Yuzuru Itoh / Anas Khawaja / Ivan Laptev / Miriam Cipullo / Ilian Atanassov / Petr Sergiev / Joanna Rorbach / Alexey Amunts / |
PubMed 要旨 | Mitoribosomes are essential for the synthesis and maintenance of bioenergetic proteins. Here we use cryo-electron microscopy to determine a series of the small mitoribosomal subunit (SSU) ...Mitoribosomes are essential for the synthesis and maintenance of bioenergetic proteins. Here we use cryo-electron microscopy to determine a series of the small mitoribosomal subunit (SSU) intermediates in complex with auxiliary factors, revealing a sequential assembly mechanism. The methyltransferase TFB1M binds to partially unfolded rRNA h45 that is promoted by RBFA, while the mRNA channel is blocked. This enables binding of METTL15 that promotes further rRNA maturation and a large conformational change of RBFA. The new conformation allows initiation factor mtIF3 to already occupy the subunit interface during the assembly. Finally, the mitochondria-specific ribosomal protein mS37 (ref. ) outcompetes RBFA to complete the assembly with the SSU-mS37-mtIF3 complex that proceeds towards mtIF2 binding and translation initiation. Our results explain how the action of step-specific factors modulate the dynamic assembly of the SSU, and adaptation of a unique protein, mS37, links the assembly to initiation to establish the catalytic human mitoribosome. |
リンク | Nature / PubMed:35676484 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.56 - 3.19 Å |
構造データ | EMDB-13551, PDB-7pnt: EMDB-13552, PDB-7pnu: EMDB-13553, PDB-7pnv: EMDB-13554, PDB-7pnw: EMDB-13555, PDB-7pnx: EMDB-13556, PDB-7pny: EMDB-13557, PDB-7pnz: EMDB-13558, PDB-7po0: EMDB-13559, PDB-7po1: EMDB-13560, PDB-7po2: EMDB-13561, PDB-7po3: EMDB-13562, PDB-7po4: |
化合物 | ChemComp-MG: ChemComp-K: ChemComp-ZN: ChemComp-FES: ChemComp-ATP: ChemComp-HOH: ChemComp-SAH: ChemComp-NAD: ChemComp-GDP: ChemComp-FME: ChemComp-GTP: ChemComp-8Q1: |
由来 |
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キーワード | RIBOSOME / Assembly intermediate / maturation / biogenesis / small subunit / mitochondrion / Initiation factor 3 / translation |