EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of adenylyl cyclase 9 activation.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 1045, Year 2022
掲載日	2022年2月24日
著者	Chao Qi / Pia Lavriha / Ved Mehta / Basavraj Khanppnavar / Inayathulla Mohammed / Yong Li / Michalis Lazaratos / Jonas V Schaefer / Birgit Dreier / Andreas Plückthun / Ana-Nicoleta Bondar / Carmen W Dessauer / Volodymyr M Korkhov /
PubMed 要旨	Adenylyl cyclase 9 (AC9) is a membrane-bound enzyme that converts ATP into cAMP. The enzyme is weakly activated by forskolin, fully activated by the G protein Gαs subunit and is autoinhibited by the ...Adenylyl cyclase 9 (AC9) is a membrane-bound enzyme that converts ATP into cAMP. The enzyme is weakly activated by forskolin, fully activated by the G protein Gαs subunit and is autoinhibited by the AC9 C-terminus. Although our recent structural studies of the AC9-Gαs complex provided the framework for understanding AC9 autoinhibition, the conformational changes that AC9 undergoes in response to activator binding remains poorly understood. Here, we present the cryo-EM structures of AC9 in several distinct states: (i) AC9 bound to a nucleotide inhibitor MANT-GTP, (ii) bound to an artificial activator (DARPin C4) and MANT-GTP, (iii) bound to DARPin C4 and a nucleotide analogue ATPαS, (iv) bound to Gαs and MANT-GTP. The artificial activator DARPin C4 partially activates AC9 by binding at a site that overlaps with the Gαs binding site. Together with the previously observed occluded and forskolin-bound conformations, structural comparisons of AC9 in the four conformations described here show that secondary structure rearrangements in the region surrounding the forskolin binding site are essential for AC9 activation.
リンク	Nat Commun / PubMed:35210418 / PubMed Central
手法	EM (単粒子)
解像度	3.8 - 4.9 Å
構造データ	13330 7pd4 EMDB-13330, PDB-7pd4: structure of Adenylyl cyclase 9 in complex with MANT-GTP 手法: EM (単粒子) / 解像度: 4.9 Å 13331 7pd8 EMDB-13331, PDB-7pd8: Structure of Adenylyl cyclase 9 in complex with DARPin C4 and MANT-GTP 手法: EM (単粒子) / 解像度: 4.2 Å 13334 7pdd EMDB-13334, PDB-7pdd: Focus refinement of soluble domain of Adenylyl cyclase 9 in complex with DARPin C4 and MANT-GTP 手法: EM (単粒子) / 解像度: 4.2 Å 13335 7pde EMDB-13335, PDB-7pde: Structure of Adenylyl cyclase 9 in complex with Gs protein alpha subunit and MANT-GTP 手法: EM (単粒子) / 解像度: 4.5 Å 13336 7pdf EMDB-13336, PDB-7pdf: focus refinement of soluble domain of adenylyl cyclase 9 in complex with Gs protein alpha subunit and MANT-GTP 手法: EM (単粒子) / 解像度: 3.8 Å 13337 7pdg EMDB-13337, PDB-7pdg: structure of adenylyl cyclase 9 in complex with DARPin C4 and ATP-aS 手法: EM (単粒子) / 解像度: 4.3 Å 13338 7pdh EMDB-13338, PDB-7pdh: structure of adenylyl cyclase 9 in complex with DARPin C4 and ATP-aS 手法: EM (単粒子) / 解像度: 4.0 Å
化合物	ChemComp-GSP: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / GTP-γ-S ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	bos taurus (ウシ) synthetic construct (人工物)
キーワード	SIGNALING PROTEIN / membrane protein / adenylyl cyclase / signalling transduction.