EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of mitochondrial translation.
ジャーナル・号・ページ	Elife, Vol. 9, Year 2020
掲載日	2020年8月19日
著者	Shintaro Aibara / Vivek Singh / Angelika Modelska / Alexey Amunts /
PubMed 要旨	Translation of mitochondrial messenger RNA (mt-mRNA) is performed by distinct mitoribosomes comprising at least 36 mitochondria-specific proteins. How these mitoribosomal proteins assist in the ...Translation of mitochondrial messenger RNA (mt-mRNA) is performed by distinct mitoribosomes comprising at least 36 mitochondria-specific proteins. How these mitoribosomal proteins assist in the binding of mt-mRNA and to what extent they are involved in the translocation of transfer RNA (mt-tRNA) is unclear. To visualize the process of translation in human mitochondria, we report ~3.0 Å resolution structure of the human mitoribosome, including the L7/L12 stalk, and eight structures of its functional complexes with mt-mRNA, mt-tRNAs, recycling factor and additional trans factors. The study reveals a transacting protein module LRPPRC-SLIRP that delivers mt-mRNA to the mitoribosomal small subunit through a dedicated platform formed by the mitochondria-specific protein mS39. Mitoribosomal proteins of the large subunit mL40, mL48, and mL64 coordinate translocation of mt-tRNA. The comparison between those structures shows dynamic interactions between the mitoribosome and its ligands, suggesting a sequential mechanism of conformational changes.
リンク	Elife / PubMed:32812867 / PubMed Central
手法	EM (単粒子)
解像度	3.5 - 5.0 Å
構造データ	11390 6zs9 EMDB-11390, PDB-6zs9: Human mitochondrial ribosome in complex with ribosome recycling factor 手法: EM (単粒子) / 解像度: 4.0 Å 11391 6zsa EMDB-11391, PDB-6zsa: Human mitochondrial ribosome bound to mRNA, A-site tRNA and P-site tRNA 手法: EM (単粒子) / 解像度: 4.0 Å 11392 6zsb EMDB-11392, PDB-6zsb: Human mitochondrial ribosome in complex with mRNA and P-site tRNA 手法: EM (単粒子) / 解像度: 4.5 Å 11393 6zsc EMDB-11393, PDB-6zsc: Human mitochondrial ribosome in complex with E-site tRNA 手法: EM (単粒子) / 解像度: 3.5 Å 11394 6zsd EMDB-11394, PDB-6zsd: Human mitochondrial ribosome in complex with mRNA, P-site tRNA and E-site tRNA 手法: EM (単粒子) / 解像度: 3.7 Å 11395 6zse EMDB-11395, PDB-6zse: Human mitochondrial ribosome in complex with mRNA, A/P-tRNA and P/E-tRNA 手法: EM (単粒子) / 解像度: 5.0 Å 11397 6zsg EMDB-11397, PDB-6zsg: Human mitochondrial ribosome in complex with mRNA, A-site tRNA, P-site tRNA and E-site tRNA 手法: EM (単粒子) / 解像度: 4.0 Å 12877 7og4 EMDB-12877, PDB-7og4: Human mitochondrial ribosome in complex with P/E-tRNA 手法: EM (単粒子) / 解像度: 3.8 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-DOL: 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE / ダルホプリスチン / 抗生剤YM / Dalfopristin ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-H8Q: ~{N}-[(3~{S},6~{R},12~{R},15~{S},16~{S},19~{S},22~{S},25~{S})-25-[[(3~{S})-1-azabicyclo[2.2.2]octan-3-yl]sulfanylmethyl]-3-[[4-(dimethylamino)phenyl]methyl]-12-ethyl-4,16-dimethyl-2,5,11,14,18,21,24-heptakis(oxidanylidene)-19-phenyl-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.0^{6,10}]hexacosan-15-yl]-3-oxidanyl-pyridine-2-carboxamide / 抗生剤*YM / Quinupristin
由来	homo sapiens (ヒト) synthetic construct (人工物) Human (ヒト)
キーワード	TRANSLATION (翻訳 (生物学)) / Mitochondria (ミトコンドリア) / Ribosome (リボソーム) / mRNA (伝令RNA) / tRNA (転移RNA)