タイトル | Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production. |
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ジャーナル・号・ページ | Nat Commun, Vol. 12, Page 5300-5300, Year 2021 |
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掲載日 | 2021年2月5日 (構造データの登録日) |
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著者 | Saaret, A. / Villiers, B. / Stricher, F. / Anissimova, M. / Cadillon, M. / Spiess, R. / Hay, S. / Leys, D. |
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リンク | Nat Commun / PubMed:34489427 |
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手法 | X線回折 |
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解像度 | 1.1 - 1.77 Å |
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構造データ | PDB-7ney: Structure of T. atroviride Fdc wild-type (TaFdc) in complex with prFMN 手法: X-RAY DIFFRACTION / 解像度: 1.74 Å PDB-7nf0: T. atroviride Fdc variant TaFdcV in complex with hydroxylated prFMN 手法: X-RAY DIFFRACTION / 解像度: 1.35 Å PDB-7nf1: Structure of T. atroviride variant TaFdcV in complex with prFMN-butynoic acid adduct 手法: X-RAY DIFFRACTION / 解像度: 1.77 Å PDB-7nf2: Structure of T. atroviride Fdc variant TaFdcV in complex with prFMN crotonic acid adduct 手法: X-RAY DIFFRACTION / 解像度: 1.33 Å PDB-7nf3: Structure of A. niger Fdc T395M variant (AnFdcI) in complex with prFMN 手法: X-RAY DIFFRACTION / 解像度: 1.1 Å PDB-7nf4: Structure of A. niger Fdc T395M R435P P438W variant (AnFdcII) in complex with prFMN 手法: X-RAY DIFFRACTION / 解像度: 1.69 Å |
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化合物 | ChemComp-4LU: 1-deoxy-5-O-phosphono-1-(3,3,4,5-tetramethyl-9,11-dioxo-2,3,8,9,10,11-hexahydro-7H-quinolino[1,8-fg]pteridin-12-ium-7-y
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由来 | - hypocrea atroviridis (strain atcc 20476 / imi 206040) (菌類)
- aspergillus niger (strain cbs 513.88 / fgsc a1513) (黒麹菌)
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キーワード | LYASE / decarboxylase / prFMN / fdc / isobutene production |
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