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-Structure paper
タイトル | Structural insights into the Venus flytrap mechanosensitive ion channel Flycatcher1. |
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ジャーナル・号・ページ | Nat Commun, Vol. 13, Issue 1, Page 850, Year 2022 |
掲載日 | 2022年2月14日 |
著者 | Sebastian Jojoa-Cruz / Kei Saotome / Che Chun Alex Tsui / Wen-Hsin Lee / Mark S P Sansom / Swetha E Murthy / Ardem Patapoutian / Andrew B Ward / |
PubMed 要旨 | Flycatcher1 (FLYC1), a MscS homolog, has recently been identified as a candidate mechanosensitive (MS) ion channel involved in Venus flytrap prey recognition. FLYC1 is a larger protein and its ...Flycatcher1 (FLYC1), a MscS homolog, has recently been identified as a candidate mechanosensitive (MS) ion channel involved in Venus flytrap prey recognition. FLYC1 is a larger protein and its sequence diverges from previously studied MscS homologs, suggesting it has unique structural features that contribute to its function. Here, we characterize FLYC1 by cryo-electron microscopy, molecular dynamics simulations, and electrophysiology. Akin to bacterial MscS and plant MSL1 channels, we find that FLYC1 central core includes side portals in the cytoplasmic cage that regulate ion preference and conduction, by identifying critical residues that modulate channel conductance. Topologically unique cytoplasmic flanking regions can adopt 'up' or 'down' conformations, making the channel asymmetric. Disruption of an up conformation-specific interaction severely delays channel deactivation by 40-fold likely due to stabilization of the channel open state. Our results illustrate novel structural features and likely conformational transitions that regulate mechano-gating of FLYC1. |
リンク | Nat Commun / PubMed:35165281 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.4 - 2.8 Å |
構造データ | EMDB-24186, PDB-7n5d: EMDB-24187, PDB-7n5e: EMDB-24188, PDB-7n5f: EMDB-24189, PDB-7n5g: |
化合物 | ChemComp-PLM: |
由来 |
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キーワード | MEMBRANE PROTEIN / mechanically activated ion channel |