EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies.
ジャーナル・号・ページ	PLoS Biol, Vol. 19, Issue 4, Page e3001237, Year 2021
掲載日	2021年4月29日
著者	Xing Zhu / Dhiraj Mannar / Shanti S Srivastava / Alison M Berezuk / Jean-Philippe Demers / James W Saville / Karoline Leopold / Wei Li / Dimiter S Dimitrov / Katharine S Tuttle / Steven Zhou / Sagar Chittori / Sriram Subramaniam /
PubMed 要旨	The recently reported "UK variant" (B.1.1.7) of SARS-CoV-2 is thought to be more infectious than previously circulating strains as a result of several changes, including the N501Y mutation. We ...The recently reported "UK variant" (B.1.1.7) of SARS-CoV-2 is thought to be more infectious than previously circulating strains as a result of several changes, including the N501Y mutation. We present a 2.9-Å resolution cryo-electron microscopy (cryo-EM) structure of the complex between the ACE2 receptor and N501Y spike protein ectodomains that shows Y501 inserted into a cavity at the binding interface near Y41 of ACE2. This additional interaction provides a structural explanation for the increased ACE2 affinity of the N501Y mutant, and likely contributes to its increased infectivity. However, this mutation does not result in large structural changes, enabling important neutralization epitopes to be retained in the spike receptor binding domain. We confirmed this through biophysical assays and by determining cryo-EM structures of spike protein ectodomains bound to 2 representative potent neutralizing antibody fragments.
リンク	PLoS Biol / PubMed:33914735 / PubMed Central
手法	EM (単粒子)
解像度	2.66 - 3.32 Å
構造データ	23872 7mjg EMDB-23872, PDB-7mjg: Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain 手法: EM (単粒子) / 解像度: 2.81 Å 23873 7mjh EMDB-23873, PDB-7mjh: Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to VH ab8 手法: EM (単粒子) / 解像度: 2.66 Å 23874 7mji EMDB-23874, PDB-7mji: Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to VH ab8 (focused refinement of RBD and VH ab8) 手法: EM (単粒子) / 解像度: 2.81 Å 23875 7mjj EMDB-23875, PDB-7mjj: Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to Fab ab1 (class 1) 手法: EM (単粒子) / 解像度: 3.32 Å 23876 7mjk EMDB-23876, PDB-7mjk: Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to Fab ab1 (class 2) 手法: EM (単粒子) / 解像度: 2.73 Å 23877 7mjl EMDB-23877, PDB-7mjl: Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to Fab ab1 (focused refinement of RBD and Fab ab1) 手法: EM (単粒子) / 解像度: 2.95 Å 23878 7mjm EMDB-23878, PDB-7mjm: Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to human ACE2 ectodomain 手法: EM (単粒子) / 解像度: 2.83 Å 23879 7mjn EMDB-23879, PDB-7mjn: Cryo-EM structure of the SARS-CoV-2 N501Y mutant spike protein ectodomain bound to human ACE2 ectodomain (focused refinement of RBD and ACE2) 手法: EM (単粒子) / 解像度: 3.29 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2) synthetic construct (人工物) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / SARS-CoV-2 (SARSコロナウイルス2) / glycoprotein (糖タンパク質) / fusion protein (融合タンパク質) / VH ab8 / neutralizing antibody (中和抗体) / Viral Protein/Immune System (ウイルス性) / Viral Protein-Immune System complex (ウイルス性) / Fab ab1 / ACE2 (アンジオテンシン変換酵素2) / VIRAL PROTEIN/Hydrolase (ウイルス性) / VIRAL PROTEIN-Hydrolase complex (ウイルス性)