タイトル | Watching right and wrong nucleotide insertion captures hidden polymerase fidelity checkpoints. |
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ジャーナル・号・ページ | Nat Commun, Vol. 13, Page 3193-3193, Year 2022 |
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掲載日 | 2021年3月21日 (構造データの登録日) |
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著者 | Jamsen, J.A. / Shock, D.D. / Wilson, S.H. |
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リンク | Nat Commun / PubMed:35680862 |
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手法 | X線回折 |
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解像度 | 1.6 - 2.377 Å |
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構造データ | PDB-7m43: DNA Polymerase Lambda, TTP:At Ca2+ Ground State Ternary Complex 手法: X-RAY DIFFRACTION / 解像度: 1.978 Å PDB-7m44: DNA Polymerase Lambda, TTP:At Mg2+ Reaction State Ternary Complex, 90 sec 手法: X-RAY DIFFRACTION / 解像度: 1.901 Å PDB-7m45: DNA Polymerase Lambda, TTP:At Mg2+ Reaction State Ternary Complex, 120 sec 手法: X-RAY DIFFRACTION / 解像度: 1.889 Å PDB-7m46: DNA Polymerase Lambda, TTP:At Mg2+ Product State Ternary Complex, 5 min 手法: X-RAY DIFFRACTION / 解像度: 1.92 Å PDB-7m47: DNA Polymerase Lambda, TTP:At Mg2+ Product State Ternary Complex, 60 min 手法: X-RAY DIFFRACTION / 解像度: 1.648 Å PDB-7m48: DNA Polymerase Lambda, TTP:At Mg2+ Product State Ternary Complex, 960 min 手法: X-RAY DIFFRACTION / 解像度: 1.93 Å PDB-7m49: DNA Polymerase Lambda, TTP:At Mn2+ Reaction State Ternary Complex, 5 min 手法: X-RAY DIFFRACTION / 解像度: 1.6 Å PDB-7m4a: DNA Polymerase Lambda, TTP:At Mn2+ Product State Ternary Complex, 20 min 手法: X-RAY DIFFRACTION / 解像度: 1.868 Å PDB-7m4b: DNA Polymerase Lambda, TTP:At Mn2+ Product State Ternary Complex, 60 min 手法: X-RAY DIFFRACTION / 解像度: 1.66 Å PDB-7m4c: DNA Polymerase Lambda, TTP:At Mn2+ Product State Ternary Complex, 960 min 手法: X-RAY DIFFRACTION / 解像度: 1.95 Å PDB-7m4d: DNA Polymerase Lambda, dCTP:At Ca2+ Ground State Ternary Complex 手法: X-RAY DIFFRACTION / 解像度: 1.817 Å PDB-7m4e: DNA Polymerase Lambda, dCTP:At Mg2+ Reaction State Ternary Complex, 120 min 手法: X-RAY DIFFRACTION / 解像度: 1.903 Å PDB-7m4f: DNA Polymerase Lambda, dCTP:At Mg2+ Product State Ternary Complex, 300 min 手法: X-RAY DIFFRACTION / 解像度: 1.947 Å PDB-7m4g: DNA Polymerase Lambda, dCTP:At Mg2+ Product State Ternary Complex, 960 min 手法: X-RAY DIFFRACTION / 解像度: 1.88 Å PDB-7m4h: DNA Polymerase Lambda, dCTP:At Mn2+ Reaction State Ternary Complex, 225 min 手法: X-RAY DIFFRACTION / 解像度: 1.923 Å PDB-7m4i: DNA Polymerase Lambda, dCTP:At Mn2+ Product State Ternary Complex, 420 min 手法: X-RAY DIFFRACTION / 解像度: 2.005 Å PDB-7m4j: DNA Polymerase Lambda, dCTP:At Mn2+ Product State Ternary Complex, 960 min 手法: X-RAY DIFFRACTION / 解像度: 2.377 Å PDB-7m4k: DNA Polymerase Lambda, TTPaS:At Ca2+ Ground State Ternary Complex 手法: X-RAY DIFFRACTION / 解像度: 1.719 Å PDB-7m4l: DNA Polymerase Lambda, TTPaS:At Mn2+ Product State Ternary Complex, 60 min 手法: X-RAY DIFFRACTION / 解像度: 1.701 Å |
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化合物 | ChemComp-YQS: [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-sulfanyl-phosphoryl] phosphono hydrogen phosphate
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由来 | - homo sapiens (ヒト)
- synthetic construct (人工物)
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キーワード | REPLICATION / Time-Lapse Crystallography / DNA Polymerase Lambda / Double Strand Break Repair / DNA Synthesis Fidelity |
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