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-Structure paper
タイトル | Structure and gating mechanism of the α7 nicotinic acetylcholine receptor. |
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ジャーナル・号・ページ | Cell, Vol. 184, Issue 8, Page 2121-2134.e13, Year 2021 |
掲載日 | 2021年4月15日 |
![]() | Colleen M Noviello / Anant Gharpure / Nuriya Mukhtasimova / Rico Cabuco / Leah Baxter / Dominika Borek / Steven M Sine / Ryan E Hibbs / ![]() |
PubMed 要旨 | The α7 nicotinic acetylcholine receptor plays critical roles in the central nervous system and in the cholinergic inflammatory pathway. This ligand-gated ion channel assembles as a homopentamer, is ...The α7 nicotinic acetylcholine receptor plays critical roles in the central nervous system and in the cholinergic inflammatory pathway. This ligand-gated ion channel assembles as a homopentamer, is exceptionally permeable to Ca, and desensitizes faster than any other Cys-loop receptor. The α7 receptor has served as a prototype for the Cys-loop superfamily yet has proven refractory to structural analysis. We present cryo-EM structures of the human α7 nicotinic receptor in a lipidic environment in resting, activated, and desensitized states, illuminating the principal steps in the gating cycle. The structures also reveal elements that contribute to its function, including a C-terminal latch that is permissive for channel opening, and an anionic ring in the extracellular vestibule that contributes to its high conductance and calcium permeability. Comparisons among the α7 structures provide a foundation for mapping the gating cycle and reveal divergence in gating mechanisms in the Cys-loop receptor superfamily. |
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手法 | EM (単粒子) |
解像度 | 2.7 - 3.6 Å |
構造データ | EMDB-22979, PDB-7koo: EMDB-22980, PDB-7koq: EMDB-22983, PDB-7kox: |
化合物 | ![]() ChemComp-NAG: ![]() ChemComp-CA: ![]() ChemComp-EPJ: ![]() ChemComp-HOH: |
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![]() | MEMBRANE PROTEIN / Cys-loop receptor |