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タイトル | Development of neutralizing antibodies against SARS-CoV-2, using a high-throughput single-B-cell cloning method. |
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ジャーナル・号・ページ | Antib Ther, Vol. 6, Issue 2, Page 76-86, Year 2023 |
掲載日 | 2023年2月17日 |
著者 | Yang Dou / Ke Xu / Yong-Qiang Deng / Zijing Jia / Jun Lan / Xiaoyu Xu / Guorui Zhang / Tianshu Cao / Pan Liu / Xiangxi Wang / Xinquan Wang / Lingjie Xu / Pan Du / Cheng-Feng Qin / Hong Liu / Yafeng Li / Guizhen Wu / Kang Wang / Bai Lu / |
PubMed 要旨 | BACKGROUND: Rapid and efficient strategies are needed to discover neutralizing antibodies (nAbs) from B cells derived from virus-infected patients.手法: Here, we report a high-throughput single-B- ...BACKGROUND: Rapid and efficient strategies are needed to discover neutralizing antibodies (nAbs) from B cells derived from virus-infected patients. 手法: Here, we report a high-throughput single-B-cell cloning method for high-throughput isolation of nAbs targeting diverse epitopes on the SARS-CoV-2-RBD (receptor binding domain) from convalescent COVID-19 patients. This method is simple, fast and highly efficient in generating SARS-CoV-2-neutralizing antibodies from COVID-19 patients' B cells. RESULTS: Using this method, we have developed multiple nAbs against distinct SARS-CoV-2-RBD epitopes. CryoEM and crystallography revealed precisely how they bind RBD. In live virus assay, these nAbs are effective in blocking viral entry to the host cells. CONCLUSION: This simple and efficient method may be useful in developing human therapeutic antibodies for other diseases and next pandemic. |
リンク | Antib Ther / PubMed:37077472 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.3 - 6.1 Å |
構造データ | EMDB-31434, PDB-7f3q: EMDB-31435: SARS-CoV-2 S trimer in complex with 2 A5-10 Fabs (2 RBDs close and 1 RBD open) EMDB-31436: SARS-CoV-2 S trimer in complex with 1 A5-10 Fab (3 RBDs close) EMDB-31437: SARS-CoV-2 S trimer in complex with 3 A34-2 Fabs (3 RBDs open) EMDB-31438: SARS-CoV-2 S trimer in complex with 2 A34-2 Fabs (2 RBDs open and 1 RBD close) EMDB-31439: SARS-CoV-2 S trimer in complex with 3 A5-10 Fabs (2 RBDs close and 1 RBD open) |
化合物 | ChemComp-NAG: ChemComp-MES: |
由来 |
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キーワード | VIRAL PROTEIN/IMMUNE SYSTEM / SARS-CoV-2 / RBD / Fab / Cocktail / VIRUS / VIRAL PROTEIN-IMMUNE SYSTEM complex |