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-Structure paper
タイトル | A Dynamic Substrate Pool Revealed by cryo-EM of a Lipid-Preserved Respiratory Supercomplex. |
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ジャーナル・号・ページ | Antioxid Redox Signal, Year 2022 |
掲載日 | 2022年4月27日 |
著者 | Tae Jin Jeon / Seong-Gyu Lee / Suk Hyun Yoo / Myeongbin Kim / Dabin Song / Joonghyun Ryu / Hwangseo Park / Deok-Soo Kim / Jaekyung Hyun / Ho Min Kim / Seong Eon Ryu / |
PubMed 要旨 | Mitochondrial respiratory supercomplexes mediate redox electron transfer, generating a proton gradient for ATP synthesis. To provide structural information on the function of supercomplexes in ... Mitochondrial respiratory supercomplexes mediate redox electron transfer, generating a proton gradient for ATP synthesis. To provide structural information on the function of supercomplexes in physiologically relevant conditions, we conducted cryoelectron microscopy studies with supercomplexes in a lipid-preserving state. Here, we present cryoelectron microscopy structures of bovine respiratory supercomplex IIIIIV by using a lipid-preserving sample preparation. The preparation greatly enhances the intercomplex quinone transfer activity. The structures reveal large intercomplex motions that result in different shapes and sizes of the intercomplex space between complexes I and III, forming a dynamic substrate pool. Biochemical and structural analyses indicated that intercomplex phospholipids mediate the intercomplex motions. An analysis of the different classes of focus-refined complex I showed that structural switches due to quinone reduction led to the formation of a novel channel that could transfer reduced quinones to the intercomplex substrate pool. Our results indicate potential mechanism for the facilitated electron transfer involving a dynamic substrate pool and intercomplex movement by which supercomplexes play an active role in the regulation of metabolic flux and reactive oxygen species. |
リンク | Antioxid Redox Signal / PubMed:34913730 |
手法 | EM (単粒子) |
解像度 | 3.8 - 8.3 Å |
構造データ | EMDB-30673, PDB-7dgq: EMDB-30674, PDB-7dgr: EMDB-30675, PDB-7dgs: EMDB-30676, PDB-7dgz: EMDB-30677, PDB-7dh0: EMDB-30706, PDB-7dkf: |
化合物 | ChemComp-PC1: ChemComp-3PE: ChemComp-FES: ChemComp-CDL: ChemComp-FMN: ChemComp-SF4: ChemComp-CU: ChemComp-HEA: ChemComp-MG: ChemComp-ZN: ChemComp-NAP: ChemComp-HEM: ChemComp-HEC: ChemComp-UQ2: |
由来 |
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キーワード | OXIDOREDUCTASE / respiratory / electron transport / Complex I |