EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A ferredoxin bridge connects the two arms of plant mitochondrial complex I.
ジャーナル・号・ページ	Plant Cell, Vol. 33, Issue 6, Page 2072-2091, Year 2021
掲載日	2021年7月19日
著者	Niklas Klusch / Jennifer Senkler / Özkan Yildiz / Werner Kühlbrandt / Hans-Peter Braun /
PubMed 要旨	Mitochondrial complex I is the main site for electron transfer to the respiratory chain and generates much of the proton gradient across the inner mitochondrial membrane. Complex I is composed of two ...Mitochondrial complex I is the main site for electron transfer to the respiratory chain and generates much of the proton gradient across the inner mitochondrial membrane. Complex I is composed of two arms, which form a conserved L-shape. We report the structures of the intact, 47-subunit mitochondrial complex I from Arabidopsis thaliana and the 51-subunit complex I from the green alga Polytomella sp., both at around 2.9 Å resolution. In both complexes, a heterotrimeric γ-carbonic anhydrase domain is attached to the membrane arm on the matrix side. Two states are resolved in A. thaliana complex I, with different angles between the two arms and different conformations of the ND1 (NADH dehydrogenase subunit 1) loop near the quinol binding site. The angle appears to depend on a bridge domain, which links the peripheral arm to the membrane arm and includes an unusual ferredoxin. We propose that the bridge domain participates in regulating the activity of plant complex I.
リンク	Plant Cell / PubMed:33768254 / PubMed Central
手法	EM (単粒子)
解像度	2.88 - 3.72 Å
構造データ	11872 7aqq EMDB-11872, PDB-7aqq: Cryo-EM structure of Arabidopsis thaliana Complex-I (membrane core) 手法: EM (単粒子) / 解像度: 3.06 Å 11873 7aqr EMDB-11873, PDB-7aqr: Cryo-EM structure of Arabidopsis thaliana Complex-I (peripheral arm) 手法: EM (単粒子) / 解像度: 2.91 Å 11874 7aqw EMDB-11874, PDB-7aqw: Cryo-EM structure of Arabidopsis thaliana Complex-I (membrane tip) 手法: EM (単粒子) / 解像度: 3.17 Å 11875 7ar7 EMDB-11875, PDB-7ar7: Cryo-EM structure of Arabidopsis thaliana complex-I (open conformation) 手法: EM (単粒子) / 解像度: 3.72 Å 11876 7ar8 EMDB-11876, PDB-7ar8: Cryo-EM structure of Arabidopsis thaliana complex-I (closed conformation) 手法: EM (単粒子) / 解像度: 3.53 Å 11877 7ar9 EMDB-11877, PDB-7ar9: Cryo-EM structure of Polytomella Complex-I (membrane arm) 手法: EM (単粒子) / 解像度: 2.97 Å 11878 7arb EMDB-11878, PDB-7arb: Cryo-EM structure of Arabidopsis thaliana Complex-I (complete composition) 手法: EM (単粒子) / 解像度: 3.41 Å 11879 7arc EMDB-11879, PDB-7arc: Cryo-EM structure of Polytomella Complex-I (peripheral arm) 手法: EM (単粒子) / 解像度: 2.88 Å 11880 7ard EMDB-11880, PDB-7ard: Cryo-EM structure of Polytomella Complex-I (complete composition) 手法: EM (単粒子) / 解像度: 3.11 Å
化合物	ChemComp-UQ9: Ubiquinone-9 ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM ChemComp-LMN: Lauryl Maltose Neopentyl Glycol / 可溶化剤YM ChemComp-FE: Unknown entry ChemComp-PC7: (7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE / リン脂質YM ChemComp-ZN: Unknown entry ChemComp-PGT: (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE / リン脂質YM ChemComp-PSF: 1,2-DICAPROYL-SN-PHOSPHATIDYL-L-SERINE / 1,2-ジカプロイル-sn-ホスファチジル-L-セリン ChemComp-T7X: Phosphatidylinositol ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-FMN: FLAVIN MONONUCLEOTIDE / FMN ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH ChemComp-8Q1: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-HOH*: WATER
由来	arabidopsis thaliana (シロイヌナズナ) Mouse-ear cress (シロイヌナズナ) thale cress (シロイヌナズナ) polytomella sp. pringsheim 198.80 (植物)
キーワード	ELECTRON TRANSPORT / Complex-I / Complex-I Arabidopsis