EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of the complete poxvirus transcription initiation process.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 28, Issue 10, Page 779-788, Year 2021
掲載日	2021年9月23日
著者	Clemens Grimm / Julia Bartuli / Bettina Boettcher / Aladar A Szalay / Utz Fischer /
PubMed 要旨	Poxviruses express their genes in the cytoplasm of infected cells using a virus-encoded multi-subunit polymerase (vRNAP) and unique transcription factors. We present cryo-EM structures that uncover ...Poxviruses express their genes in the cytoplasm of infected cells using a virus-encoded multi-subunit polymerase (vRNAP) and unique transcription factors. We present cryo-EM structures that uncover the complete transcription initiation phase of the poxvirus vaccinia. In the pre-initiation complex, the heterodimeric early transcription factor VETFs/l adopts an arc-like shape spanning the polymerase cleft and anchoring upstream and downstream promoter elements. VETFI emerges as a TBP-like protein that inserts asymmetrically into the DNA major groove, triggers DNA melting, ensures promoter recognition and enforces transcription directionality. The helicase VETFs fosters promoter melting and the phospho-peptide domain (PPD) of vRNAP subunit Rpo30 enables transcription initiation. An unprecedented upstream promoter scrunching mechanism assisted by the helicase NPH-I probably fosters promoter escape and transition into elongation. Our structures shed light on unique mechanisms of poxviral gene expression and aid the understanding of thus far unexplained universal principles in transcription.
リンク	Nat Struct Mol Biol / PubMed:34556871
手法	EM (単粒子)
解像度	2.7 - 3.15 Å
構造データ	11824 7amv EMDB-11824, PDB-7amv: Atomic structure of the poxvirus transcription pre-initiation complex in the initially melted state 手法: EM (単粒子) / 解像度: 2.8 Å 11843 7aof EMDB-11843, PDB-7aof: Atomic structure of the poxvirus transcription late pre-initiation complex 手法: EM (単粒子) / 解像度: 2.98 Å 11844 7aoh EMDB-11844, PDB-7aoh: Atomic structure of the poxvirus late initially transcribing complex 手法: EM (単粒子) / 解像度: 2.7 Å 11848 7aoz EMDB-11848, PDB-7aoz: Atomic structure of the poxvirus transcription initiation complex in conformation 1 手法: EM (単粒子) / 解像度: 2.85 Å 11850 7ap8 EMDB-11850, PDB-7ap8: Atomic structure of the poxvirus initially transcribing complex in conformation 2 手法: EM (単粒子) / 解像度: 3.15 Å 11851 7ap9 EMDB-11851, PDB-7ap9: Atomic structure of the poxvirus initially transcribing complex in conformation 3 手法: EM (単粒子) / 解像度: 3.01 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry
由来	vaccinia virus glv-1h68 (ウイルス) synthetic construct (人工物)
キーワード	TRANSCRIPTION / Vaccinia / Virus / RNA polymerase / DNA-dependent / pre-initiation complex / PIC / initially melted / poxvirus / poxviridae / initiation complex