EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of inhibitor-bound mammalian complex I.
ジャーナル・号・ページ	Nat Commun, Vol. 11, Issue 1, Page 5261, Year 2020
掲載日	2020年10月16日
著者	Hannah R Bridges / Justin G Fedor / James N Blaza / Andrea Di Luca / Alexander Jussupow / Owen D Jarman / John J Wright / Ahmed-Noor A Agip / Ana P Gamiz-Hernandez / Maxie M Roessler / Ville R I Kaila / Judy Hirst /
PubMed 要旨	Respiratory complex I (NADH:ubiquinone oxidoreductase) captures the free energy from oxidising NADH and reducing ubiquinone to drive protons across the mitochondrial inner membrane and power ...Respiratory complex I (NADH:ubiquinone oxidoreductase) captures the free energy from oxidising NADH and reducing ubiquinone to drive protons across the mitochondrial inner membrane and power oxidative phosphorylation. Recent cryo-EM analyses have produced near-complete models of the mammalian complex, but leave the molecular principles of its long-range energy coupling mechanism open to debate. Here, we describe the 3.0-Å resolution cryo-EM structure of complex I from mouse heart mitochondria with a substrate-like inhibitor, piericidin A, bound in the ubiquinone-binding active site. We combine our structural analyses with both functional and computational studies to demonstrate competitive inhibitor binding poses and provide evidence that two inhibitor molecules bind end-to-end in the long substrate binding channel. Our findings reveal information about the mechanisms of inhibition and substrate reduction that are central for understanding the principles of energy transduction in mammalian complex I.
リンク	Nat Commun / PubMed:33067417 / PubMed Central
手法	EM (単粒子)
解像度	3.0 - 3.1 Å
構造データ	11377 6zr2 6ztq EMDB-11377, PDB-6zr2: Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A PDB-6ztq: Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A 手法: EM (単粒子) / 解像度: 3.1 Å 11424 6zr2 6ztq EMDB-11424, PDB-6ztq: Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A PDB-6zr2: Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A 手法: EM (単粒子) / 解像度: 3.0 Å 11425 6zr2 EMDB-11425: Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A (Falcon 3) PDB-6zr2: Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM / ホスファチジルコリン ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER / 鉄・硫黄クラスター ChemComp-FMN: FLAVIN MONONUCLEOTIDE / FMN / フラビンモノヌクレオチド ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM / ホスファチジルエタノールアミン ChemComp-CDL: CARDIOLIPIN / リン脂質YM / Cardiolipin ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH / ニコチンアミドアデニンジヌクレオチドリン酸 ChemComp-ZN: Unknown entry ChemComp-EHZ: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate ChemComp-HQH: Piericidin A / ピエリシジンA / 抗生剤*YM / ピエリシジンA
由来	mus musculus (ハツカネズミ) Mouse (ネズミ)
キーワード	OXIDOREDUCTASE (酸化還元酵素) / NADH (ニコチンアミドアデニンジヌクレオチド) / ubiquinone (ユビキノン) / complex I (NADH:ユビキノン還元酵素 (水素イオン輸送型))