+検索条件
-Structure paper
タイトル | Structure of plant photosystem I-plastocyanin complex reveals strong hydrophobic interactions. |
---|---|
ジャーナル・号・ページ | Biochem J, Vol. 478, Issue 12, Page 2371-2384, Year 2021 |
掲載日 | 2021年6月25日 |
![]() | Ido Caspy / Mariia Fadeeva / Sebastian Kuhlgert / Anna Borovikova-Sheinker / Daniel Klaiman / Gal Masrati / Friedel Drepper / Nir Ben-Tal / Michael Hippler / Nathan Nelson / ![]() ![]() ![]() |
PubMed 要旨 | Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and ...Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and electron transfer between PSI and Pc. Structural data at 2.74 Å resolution reveals strong hydrophobic interactions in the plant PSI-Pc ternary complex, leading to exclusion of water molecules from PsaA-PsaB/Pc interface once the PSI-Pc complex forms. Upon oxidation of Pc, a slight tilt of bound oxidized Pc allows water molecules to accommodate the space between Pc and PSI to drive Pc dissociation. Such a scenario is consistent with the six times larger dissociation constant of oxidized as compared with reduced Pc and mechanistically explains how this molecular machine optimized electron transfer for fast turnover. |
![]() | ![]() ![]() ![]() |
手法 | EM (単粒子) |
解像度 | 2.74 Å |
構造データ | EMDB-11326, PDB-6zoo: |
化合物 | ![]() ChemComp-CL0: ![]() ChemComp-CLA: ![]() ChemComp-PQN: ![]() ChemComp-SF4: ![]() ChemComp-BCR: ![]() ChemComp-LHG: ![]() ChemComp-LMT: ![]() ChemComp-LMG: ![]() ChemComp-CA: ![]() ChemComp-DGD: ![]() ChemComp-LUT: ![]() ChemComp-CHL: ![]() ChemComp-XAT: ![]() ChemComp-3PH: ![]() ChemComp-CU: ![]() ChemComp-HOH: |
由来 |
|
![]() | PHOTOSYNTHESIS / Photosystem I / membrane protein |