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-Structure paper
タイトル | Cryo-EM structures and functional properties of CALHM channels of the human placenta. |
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ジャーナル・号・ページ | Elife, Vol. 9, Year 2020 |
掲載日 | 2020年5月6日 |
著者 | Katarzyna Drożdżyk / Marta Sawicka / Maria-Isabel Bahamonde-Santos / Zaugg Jonas / Dawid Deneka / Christiane Albrecht / Raimund Dutzler / |
PubMed 要旨 | The transport of substances across the placenta is essential for the development of the fetus. Here, we were interested in the role of channels of the calcium homeostasis modulator (CALHM) family in ...The transport of substances across the placenta is essential for the development of the fetus. Here, we were interested in the role of channels of the calcium homeostasis modulator (CALHM) family in the human placenta. By transcript analysis, we found the paralogs CALHM2, 4, and 6 to be highly expressed in this organ and upregulated during trophoblast differentiation. Based on electrophysiology, we observed that activation of these paralogs differs from the voltage- and calcium-gated channel CALHM1. Cryo-EM structures of CALHM4 display decameric and undecameric assemblies with large cylindrical pore, while in CALHM6 a conformational change has converted the pore shape into a conus that narrows at the intracellular side, thus describing distinct functional states of the channel. The pore geometry alters the distribution of lipids, which occupy the cylindrical pore of CALHM4 in a bilayer-like arrangement whereas they have redistributed in the conical pore of CALHM6 with potential functional consequences. |
リンク | Elife / PubMed:32374262 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.82 - 6.23 Å |
構造データ | EMDB-10917, PDB-6ytk: EMDB-10919, PDB-6ytl: EMDB-10920, PDB-6yto: EMDB-10921, PDB-6ytq: EMDB-10924, PDB-6ytv: EMDB-10925, PDB-6ytx: |
由来 |
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キーワード | MEMBRANE PROTEIN / calcium homeostasis modulator / ion channel / placenta |