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-Structure paper
タイトル | Structure of a human 48 translational initiation complex. |
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ジャーナル・号・ページ | Science, Vol. 369, Issue 6508, Page 1220-1227, Year 2020 |
掲載日 | 2020年9月4日 |
著者 | Jailson Brito Querido / Masaaki Sokabe / Sebastian Kraatz / Yuliya Gordiyenko / J Mark Skehel / Christopher S Fraser / V Ramakrishnan / |
PubMed 要旨 | A key step in translational initiation is the recruitment of the 43 preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to ...A key step in translational initiation is the recruitment of the 43 preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to form the 48 initiation complex (i.e., the 48). The 48 then scans along the mRNA to locate a start codon. To understand the mechanisms involved, we used cryo-electron microscopy to determine the structure of a reconstituted human 48 The structure reveals insights into early events of translation initiation complex assembly, as well as how eIF4F interacts with subunits of eIF3 near the mRNA exit channel in the 43 The location of eIF4F is consistent with a slotting model of mRNA recruitment and suggests that downstream mRNA is unwound at least in part by being "pulled" through the 40 subunit during scanning. |
リンク | Science / PubMed:32883864 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.1 - 6.0 Å |
構造データ | EMDB-10769, PDB-6ybd: EMDB-10772, PDB-6ybs: EMDB-10773, PDB-6ybt: EMDB-10774, PDB-6ybv: EMDB-10775, PDB-6ybw: EMDB-11302, PDB-6zmw: |
化合物 | ChemComp-MG: ChemComp-ZN: |
由来 |
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キーワード | TRANSLATION / eIF3 / ribosome / initiation complex |