EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
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ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of mechano-chemical coupling by the mitotic kinesin KIF14.
ジャーナル・号・ページ	Nat Commun, Vol. 12, Issue 1, Page 3637, Year 2021
掲載日	2021年6月15日
著者	Matthieu P M H Benoit / Ana B Asenjo / Mohammadjavad Paydar / Sabin Dhakal / Benjamin H Kwok / Hernando Sosa /
PubMed 要旨	KIF14 is a mitotic kinesin whose malfunction is associated with cerebral and renal developmental defects and several cancers. Like other kinesins, KIF14 couples ATP hydrolysis and microtubule binding ...KIF14 is a mitotic kinesin whose malfunction is associated with cerebral and renal developmental defects and several cancers. Like other kinesins, KIF14 couples ATP hydrolysis and microtubule binding to the generation of mechanical work, but the coupling mechanism between these processes is still not fully clear. Here we report 20 high-resolution (2.7-3.9 Å) cryo-electron microscopy KIF14-microtubule structures with complementary functional assays. Analysis procedures were implemented to separate coexisting conformations of microtubule-bound monomeric and dimeric KIF14 constructs. The data provide a comprehensive view of the microtubule and nucleotide induced KIF14 conformational changes. It shows that: 1) microtubule binding, the nucleotide species, and the neck-linker domain govern the transition between three major conformations of the motor domain; 2) an undocked neck-linker prevents the nucleotide-binding pocket to fully close and dampens ATP hydrolysis; 3) 13 neck-linker residues are required to assume a stable docked conformation; 4) the neck-linker position controls the hydrolysis rather than the nucleotide binding step; 5) the two motor domains of KIF14 dimers adopt distinct conformations when bound to the microtubule; and 6) the formation of the two-heads-bound-state introduces structural changes in both motor domains of KIF14 dimers. These observations provide the structural basis for a coordinated chemo-mechanical kinesin translocation model.
リンク	Nat Commun / PubMed:34131133 / PubMed Central
手法	EM (らせん対称)
解像度	2.7 - 3.9 Å
構造データ	21932 6wwe EMDB-21932, PDB-6wwe: Apo KIF14[391-772] in complex with a microtubule 手法: EM (らせん対称) / 解像度: 3.9 Å 21933 6wwf EMDB-21933, PDB-6wwf: KIF14[391-772] - ADP in complex with a microtubule 手法: EM (らせん対称) / 解像度: 3.3 Å 21934 6wwg EMDB-21934, PDB-6wwg: KIF14[391-772] dimer two-heads-bound state - ADP-AlFx in complex with a microtubule 手法: EM (らせん対称) / 解像度: 2.9 Å 21935 6wwh EMDB-21935, PDB-6wwh: KIF14[391-772] dimer two-heads-bound state - AMP-PNP in complex with a microtubule 手法: EM (らせん対称) / 解像度: 3.8 Å 21936 6wwi EMDB-21936, PDB-6wwi: Apo KIF14[391-755] in complex with a microtubule 手法: EM (らせん対称) / 解像度: 3.6 Å 21937 6wwj EMDB-21937, PDB-6wwj: KIF14[391-755] - ADP in complex with a microtubule 手法: EM (らせん対称) / 解像度: 3.4 Å 21938 6wwk EMDB-21938, PDB-6wwk: KIF14[391-755] dimer two-heads-bound state - ADP-AlFx in complex with a microtubule 手法: EM (らせん対称) / 解像度: 3.0 Å 21939 6wwl EMDB-21939, PDB-6wwl: KIF14[391-755] dimer two-heads-bound state - AMP-PNP in complex with a microtubule 手法: EM (らせん対称) / 解像度: 3.1 Å 21940 6wwm EMDB-21940, PDB-6wwm: KIF14[391-748] - ADP in complex with a microtubule 手法: EM (らせん対称) / 解像度: 2.8 Å 21941 6wwn EMDB-21941, PDB-6wwn: KIF14[391-748] - ADP-AlFx in complex with a microtubule 手法: EM (らせん対称) / 解像度: 3.5 Å 21942 6wwo EMDB-21942, PDB-6wwo: KIF14[391-748] - AMP-PNP in complex with a microtubule 手法: EM (らせん対称) / 解像度: 2.8 Å 21943 6wwp EMDB-21943, PDB-6wwp: Apo KIF14[391-743] in complex with a microtubule 手法: EM (らせん対称) / 解像度: 3.1 Å 21944 6wwq EMDB-21944, PDB-6wwq: KIF14[391-743] - ADP in complex with a microtubule 手法: EM (らせん対称) / 解像度: 3.0 Å 21945 6wwr EMDB-21945, PDB-6wwr: Kif14[391-743] - ADP-AlFx open state class in complex with a microtubule 手法: EM (らせん対称) / 解像度: 2.7 Å 21946 6wws EMDB-21946, PDB-6wws: Kif14[391-743] - AMP-PNP open state class in complex with a microtubule 手法: EM (らせん対称) / 解像度: 2.7 Å 21947 6wwt EMDB-21947, PDB-6wwt: Apo KIF14[391-735] in complex with a microtubule 手法: EM (らせん対称) / 解像度: 3.2 Å 21948 6wwu EMDB-21948, PDB-6wwu: KIF14[391-735] - ADP-AlFx in complex with a microtubule 手法: EM (らせん対称) / 解像度: 2.7 Å 21949 6wwv EMDB-21949, PDB-6wwv: KIF14[391-735] - ANP-PNP in complex with a microtubule 手法: EM (らせん対称) / 解像度: 3.1 Å 23540 7lvq EMDB-23540, PDB-7lvq: KIF14[391-743] - AMP-PNP closed state class in complex with a microtubule 手法: EM (らせん対称) / 解像度: 2.9 Å 23541 7lvr EMDB-23541, PDB-7lvr: KIF14[391-743] - ADP-AlFx closed state class in complex with a microtubule 手法: EM (らせん対称) / 解像度: 2.9 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-TA1: TAXOL / パクリタキセル / 薬剤, 化学療法薬YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-AF3: ALUMINUM FLUORIDE / トリフルオロアルミニウム ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体*YM
由来	mus musculus (ハツカネズミ) sus scrofa (ブタ)
キーワード	MOTOR PROTEIN / KIF14 / kinesin / motility / microtubule / tubulin