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-Structure paper
タイトル | AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex. |
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ジャーナル・号・ページ | Nat Commun, Vol. 11, Issue 1, Page 2730, Year 2020 |
掲載日 | 2020年6月1日 |
著者 | Marscha Hirschi / Wang-Ting Lu / Andrew Santiago-Frangos / Royce Wilkinson / Sarah M Golden / Alan R Davidson / Gabriel C Lander / Blake Wiedenheft / |
PubMed 要旨 | Bacteria have evolved sophisticated adaptive immune systems, called CRISPR-Cas, that provide sequence-specific protection against phage infection. In turn, phages have evolved a broad spectrum of ...Bacteria have evolved sophisticated adaptive immune systems, called CRISPR-Cas, that provide sequence-specific protection against phage infection. In turn, phages have evolved a broad spectrum of anti-CRISPRs that suppress these immune systems. Here we report structures of anti-CRISPR protein IF9 (AcrIF9) in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy). In addition to sterically blocking the hybridization of complementary dsDNA to the CRISPR RNA, our results show that AcrIF9 binding also promotes non-sequence-specific engagement with dsDNA, potentially sequestering the complex from target DNA. These findings highlight the versatility of anti-CRISPR mechanisms utilized by phages to suppress CRISPR-mediated immune systems. |
リンク | Nat Commun / PubMed:32483187 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.9 - 4.2 Å |
構造データ | EMDB-21516, PDB-6w1x: EMDB-21517: Cryo-EM structure of anti-CRISPR AcrIF9 and dsDNA, bound to the type I-F crRNA-guided CRISPR surveillance complex |
由来 |
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キーワード | IMMUNE SYSTEM/RNA / type I-F CRISPR RNA-guided surveillance complex / Csy complex / IMMUNE SYSTEM-RNA complex / IMMUNE SYSTEM/DNA/RNA / IMMUNE SYSTEM-DNA-RNA complex |