EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Distortion of the bilayer and dynamics of the BAM complex in lipid nanodiscs.
ジャーナル・号・ページ	Commun Biol, Vol. 3, Issue 1, Page 766, Year 2020
掲載日	2020年12月14日
著者	Matthew G Iadanza / Bob Schiffrin / Paul White / Matthew A Watson / Jim E Horne / Anna J Higgins / Antonio N Calabrese / David J Brockwell / Roman Tuma / Antreas C Kalli / Sheena E Radford / Neil A Ranson /
PubMed 要旨	The β-barrel assembly machinery (BAM) catalyses the folding and insertion of β-barrel outer membrane proteins (OMPs) into the outer membranes of Gram-negative bacteria by mechanisms that remain ...The β-barrel assembly machinery (BAM) catalyses the folding and insertion of β-barrel outer membrane proteins (OMPs) into the outer membranes of Gram-negative bacteria by mechanisms that remain unclear. Here, we present an ensemble of cryoEM structures of the E. coli BamABCDE (BAM) complex in lipid nanodiscs, determined using multi-body refinement techniques. These structures, supported by single-molecule FRET measurements, describe a range of motions in the BAM complex, mostly localised within the periplasmic region of the major subunit BamA. The β-barrel domain of BamA is in a 'lateral open' conformation in all of the determined structures, suggesting that this is the most energetically favourable species in this bilayer. Strikingly, the BAM-containing lipid nanodisc is deformed, especially around BAM's lateral gate. This distortion is also captured in molecular dynamics simulations, and provides direct structural evidence for the lipid 'disruptase' activity of BAM, suggested to be an important part of its functional mechanism.
リンク	Commun Biol / PubMed:33318620 / PubMed Central
手法	EM (単粒子)
解像度	6.65 - 10.8 Å
構造データ	10247 6smx EMDB-10247, PDB-6smx: BamABCDE in MSP1D1 nanodisc 手法: EM (単粒子) / 解像度: 6.65 Å 10248 6sn0 EMDB-10248, PDB-6sn0: BamABCDE in MSP1D1 nanodisc ensemble 0-1 手法: EM (単粒子) / 解像度: 10.8 Å 10249 6sn2 EMDB-10249, PDB-6sn2: BamABCDE in MSP1D1 nanodisc ensemble 0-2 手法: EM (単粒子) / 解像度: 9.5 Å 10250 6sn3 EMDB-10250, PDB-6sn3: BamABCDE in MSP1D1 nanodisc ensemble 0-3 手法: EM (単粒子) / 解像度: 8.4 Å 10251 6sn4 EMDB-10251: BamABCDE in MSP1D1 nanodisc ensemble 04 PDB-6sn4: BamABCDE in MSP1D1 nanodisc ensemble 0-4 手法: EM (単粒子) / 解像度: 9.5 Å 10252 6sn5 EMDB-10252, PDB-6sn5: BamABCDE in MSP1D1 nanodisc ensemble 0-5 手法: EM (単粒子) / 解像度: 9.8 Å 10253 6sn7 EMDB-10253, PDB-6sn7: BamABCDE in MSP1D1 nanodisc ensemble 0-6 手法: EM (単粒子) / 解像度: 8.9 Å 10254 6sn8 EMDB-10254, PDB-6sn8: BamABCDE in MSP1D1 nanodisc ensemble 0-7 手法: EM (単粒子) / 解像度: 8.4 Å 10255 6sn9 EMDB-10255, PDB-6sn9: BamABCDE in MSP1D1 nanodisc ensemble 0-8 手法: EM (単粒子) / 解像度: 9.8 Å 10268 6so7 EMDB-10268: BamABCDE in MSP1D1 nanodisc 1-2 PDB-6so7: BamABCDE in MSP1D1 nanodisc ensemble 1-2 手法: EM (単粒子) / 解像度: 10.5 Å 10269 6so8 EMDB-10269, PDB-6so8: BamABCDE in MSP1D1 nanodisc ensemble 1-3 手法: EM (単粒子) / 解像度: 9.8 Å 10270 6soa EMDB-10270, PDB-6soa: BamABCDE in MSP1D1 nanodisc ensemble 0-9 手法: EM (単粒子) / 解像度: 10.8 Å 10271 6sob EMDB-10271, PDB-6sob: BamABCDE in MSP1D1 nanodisc ensemble 1-4 手法: EM (単粒子) / 解像度: 8.5 Å 10272 6soc EMDB-10272, PDB-6soc: BamABCDE in MSP1D1 nanodisc ensemble 1-5 手法: EM (単粒子) / 解像度: 9.0 Å 10274 6sog EMDB-10274, PDB-6sog: BamABCDE in MSP1D1 nanodisc ensemble 1-6 手法: EM (単粒子) / 解像度: 8.3 Å 10275 6soh EMDB-10275, PDB-6soh: BamABCDE in MSP1D1 nanodisc ensemble 1-7 手法: EM (単粒子) / 解像度: 9.5 Å 10276 6soj EMDB-10276, PDB-6soj: BamABCDE in MSP1D1 nanodisc ensemble 1-8 手法: EM (単粒子) / 解像度: 10.4 Å
由来	Escherichia coli (大腸菌)
キーワード	MEMBRANE PROTEIN / Outer membrane / OMP / beta-barrel / folding / insertion