EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of a RAG-like transposase during cut-and-paste transposition.
ジャーナル・号・ページ	Nature, Vol. 575, Issue 7783, Page 540-544, Year 2019
掲載日	2019年11月13日
著者	Chang Liu / Yang Yang / David G Schatz /
PubMed 要旨	Transposons have had a pivotal role in genome evolution and are believed to be the evolutionary progenitors of the RAG1-RAG2 recombinase, an essential component of the adaptive immune system in jawed ...Transposons have had a pivotal role in genome evolution and are believed to be the evolutionary progenitors of the RAG1-RAG2 recombinase, an essential component of the adaptive immune system in jawed vertebrates. Here we report one crystal structure and five cryo-electron microscopy structures of Transib, a RAG1-like transposase from Helicoverpa zea, that capture the entire transposition process from the apo enzyme to the terminal strand transfer complex with transposon ends covalently joined to target DNA, at resolutions of 3.0-4.6 Å. These structures reveal a butterfly-shaped complex that undergoes two cycles of marked conformational changes in which the 'wings' of the transposase unfurl to bind substrate DNA, close to execute cleavage, open to release the flanking DNA and close again to capture and attack target DNA. Transib possesses unique structural elements that compensate for the absence of a RAG2 partner, including a loop that interacts with the transposition target site and an accordion-like C-terminal tail that elongates and contracts to help to control the opening and closing of the enzyme and assembly of the active site. Our findings reveal the detailed reaction pathway of a eukaryotic cut-and-paste transposase and illuminate some of the earliest steps in the evolution of the RAG recombinase.
リンク	Nature / PubMed:31723264 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.01 - 4.6 Å
構造データ	20452 6pqr EMDB-20452, PDB-6pqr: Cryo-EM structure of HzTransib/intact TIR substrate DNA pre-reaction complex (PRC) 手法: EM (単粒子) / 解像度: 3.4 Å 20453 6pqu EMDB-20453, PDB-6pqu: Cryo-EM structure of HzTransib/nicked TIR substrate DNA pre-reaction complex (PRC) 手法: EM (単粒子) / 解像度: 3.3 Å 20455 6pqx EMDB-20455, PDB-6pqx: Cryo-EM structure of HzTransib/nicked TIR substrate DNA hairpin forming complex (HFC) 手法: EM (単粒子) / 解像度: 4.6 Å 20456 6pqy EMDB-20456, PDB-6pqy: Cryo-EM structure of HzTransib/TIR DNA transposon end complex (TEC) 手法: EM (単粒子) / 解像度: 4.2 Å 20457 6pr5 EMDB-20457, PDB-6pr5: Cryo-EM structure of HzTransib strand transfer complex (STC) 手法: EM (単粒子) / 解像度: 3.3 Å PDB-6pqn: Crystal structure of HzTransib transposase 手法: X-RAY DIFFRACTION / 解像度: 3.01 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-PO4: PHOSPHATE ION / ホスファ-ト / リン酸塩 ChemComp-GOL: GLYCEROL / グリセロ-ル / グリセリン ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-HOH: WATER / 水 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	helicoverpa zea (蝶・蛾)
キーワード	RECOMBINATION (遺伝的組換え) / RAG-like transposase / DDE family enzyme / Transib / Evolution of RAG / recombination/dna / Terminal inverted repeat. / recombination-dna complex / Strand transfer. (逆転写酵素)