EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis.
ジャーナル・号・ページ	Sci Rep, Vol. 10, Issue 1, Page 16301, Year 2020
掲載日	2020年10月1日
著者	Eileen L Murphy / Kavindra V Singh / Bryant Avila / Torsten Kleffmann / Steven T Gregory / Barbara E Murray / Kurt L Krause / Reza Khayat / Gerwald Jogl /
PubMed 要旨	Enterococcus faecalis is a gram-positive organism responsible for serious infections in humans, but as with many bacterial pathogens, resistance has rendered a number of commonly used antibiotics ...Enterococcus faecalis is a gram-positive organism responsible for serious infections in humans, but as with many bacterial pathogens, resistance has rendered a number of commonly used antibiotics ineffective. Here, we report the cryo-EM structure of the E. faecalis 70S ribosome to a global resolution of 2.8 Å. Structural differences are clustered in peripheral and solvent exposed regions when compared with Escherichia coli, whereas functional centres, including antibiotic binding sites, are similar to other bacterial ribosomes. Comparison of intersubunit conformations among five classes obtained after three-dimensional classification identifies several rotated states. Large ribosomal subunit protein bL31, which forms intersubunit bridges to the small ribosomal subunit, assumes different conformations in the five classes, revealing how contacts to the small subunit are maintained throughout intersubunit rotation. A tRNA observed in one of the five classes is positioned in a chimeric pe/E position in a rotated ribosomal state. The 70S ribosome structure of E. faecalis now extends our knowledge of bacterial ribosome structures and may serve as a basis for the development of novel antibiotic compounds effective against this pathogen.
リンク	Sci Rep / PubMed:33004869 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 4.14 Å
構造データ	0656 6o8w EMDB-0656, PDB-6o8w: Cryo-EM image reconstruction of the 70S Ribosome Enterococcus faecalis Class01 手法: EM (単粒子) / 解像度: 3.53 Å 0657 6o8x EMDB-0657, PDB-6o8x: Cryo-EM image reconstruction of the 70S Ribosome Enterococcus faecalis Class02 手法: EM (単粒子) / 解像度: 3.68 Å 0658 6o8y EMDB-0658, PDB-6o8y: Cryo-EM image reconstruction of the 70S Ribosome Enterococcus faecalis Class03 手法: EM (単粒子) / 解像度: 4.14 Å 0659 6o8z EMDB-0659, PDB-6o8z: Cryo-EM image reconstruction of the 70S Ribosome Enterococcus faecalis Class04 手法: EM (単粒子) / 解像度: 3.49 Å 0660 6o90 EMDB-0660, PDB-6o90: Cryo-EM image reconstruction of the 70S Ribosome Enterococcus faecalis Class05 手法: EM (単粒子) / 解像度: 3.49 Å 21562 6w6p EMDB-21562, PDB-6w6p: MultiBody Refinement of 70S Ribosome from Enterococcus faecalis 手法: EM (単粒子) / 解像度: 2.9 Å 21907 6wu9 EMDB-21907, PDB-6wu9: 50S subunit of 70S Ribosome Enterococcus faecalis MultiBody refinement 手法: EM (単粒子) / 解像度: 2.9 Å 21908 6wua EMDB-21908, PDB-6wua: 30S subunit (head) of 70S Ribosome Enterococcus faecalis MultiBody refinement 手法: EM (単粒子) / 解像度: 3.2 Å 21909 6wub EMDB-21909, PDB-6wub: 30S subunit (head) of 70S Ribosome Enterococcus faecalis MultiBody refinement 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-ZN: Unknown entry
由来	enterococcus faecalis (乳酸球菌) enterococcus faecalis og1rf (乳酸球菌)
キーワード	RIBOSOME (リボソーム) / 70S / pathogen (病原体) / antibiotic development / antibiotic resistant (抗微生物薬耐性)