EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structure of circumsporozoite protein with a vaccine-elicited antibody is stabilized by somatically mutated inter-Fab contacts.
ジャーナル・号・ページ	Sci Adv, Vol. 4, Issue 10, Page eaau8529, Year 2018
掲載日	2018年10月10日
著者	David Oyen / Jonathan L Torres / Christopher A Cottrell / C Richter King / Ian A Wilson / Andrew B Ward /
PubMed 要旨	The circumsporozoite protein (CSP) on the surface of sporozoites is important for parasite development, motility, and host hepatocyte invasion. However, intrinsic disorder of the NANP repeat ...The circumsporozoite protein (CSP) on the surface of sporozoites is important for parasite development, motility, and host hepatocyte invasion. However, intrinsic disorder of the NANP repeat sequence in the central region of CSP has hindered its structural and functional characterization. Here, the cryo-electron microscopy structure at ~3.4-Å resolution of a recombinant shortened CSP construct with the variable domains (Fabs) of a highly protective monoclonal antibody reveals an extended spiral conformation of the central NANP repeat region surrounded by antibodies. This unusual structure appears to be stabilized and/or induced by interaction with an antibody where contacts between adjacent Fabs are somatically mutated and enhance the interaction. This maturation in non-antigen contact residues may be an effective mechanism for antibodies to target tandem repeat sequences and provide novel insights into malaria vaccine design.
リンク	Sci Adv / PubMed:30324137 / PubMed Central
手法	EM (単粒子)
解像度	3.37 - 27.0 Å
構造データ	EMDB-7897: Full-length Plasmodium falciparum Circumsporozoite Protein (CSP) in complex with fragment antigen binding (Fab) 311. 手法: EM (単粒子) / 解像度: 27.0 Å EMDB-7898: recombinant, shortened Plasmodium falciparum Circumsporozoite Protein (CSP) in complex with immunoglobulin g (IgG) 311 . 手法: EM (単粒子) / 解像度: 27.0 Å EMDB-7899: Recombinant, shortened Plasmodium falciparum Cisrcumsporozoite Protein (rsCSP) in complex with fragment antigen binding (Fab) 317 手法: EM (単粒子) / 解像度: 14.0 Å 9065 6mb3 EMDB-9065, PDB-6mb3: Cryo-EM structure of the circumsporozoite protein of Plasmodium falciparum with a vaccine-elicited antibody reveals maturation of inter-antibody contacts 手法: EM (単粒子) / 解像度: 3.37 Å 9114 6mhg EMDB-9114, PDB-6mhg: Cryo-EM structure of the circumsporozoite protein of Plasmodium falciparum with a vaccine-elicited antibody reveals maturation of inter-antibody contacts 手法: EM (単粒子) / 解像度: 3.57 Å
由来	plasmodium falciparum (マラリア病原虫) homo sapiens (ヒト)
キーワード	IMMUNE SYSTEM / Plasmodium falciparum / antibody / CSP / rsCSP