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-Structure paper
タイトル | Machining protein microcrystals for structure determination by electron diffraction. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 115, Issue 38, Page 9569-9573, Year 2018 |
掲載日 | 2018年9月18日 |
著者 | Helen M E Duyvesteyn / Abhay Kotecha / Helen M Ginn / Corey W Hecksel / Emma V Beale / Felix de Haas / Gwyndaf Evans / Peijun Zhang / Wah Chiu / David I Stuart / |
PubMed 要旨 | We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure ...We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffraction and micron scale of synchrotron microfocus beamlines. The demonstration that atomic information can be retained suggests that milling could provide such detail on sections cut from vitrified cells. |
リンク | Proc Natl Acad Sci U S A / PubMed:30171169 / PubMed Central |
手法 | EM (電子線結晶学) |
解像度 | 1.9 Å |
構造データ | PDB-6h3b: |
化合物 | ChemComp-CL: ChemComp-HOH: |
由来 |
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キーワード | HYDROLASE / lysozyme |