タイトル | Time-lapse crystallography snapshots of a double-strand break repair polymerase in action. |
---|
ジャーナル・号・ページ | Nat Commun, Vol. 8, Page 253-253, Year 2017 |
---|
掲載日 | 2016年11月17日 (構造データの登録日) |
---|
著者 | Jamsen, J.A. / Beard, W.A. / Pedersen, L.C. / Shock, D.D. / Moon, A.F. / Krahn, J.M. / Bebenek, K. / Kunkel, T.A. / Wilson, S.H. |
---|
リンク | Nat Commun / PubMed:28811466 |
---|
手法 | X線回折 |
---|
解像度 | 1.651 - 2.101 Å |
---|
構造データ | PDB-5txx: DNA Polymerase Mu Pre-Catalytic Ground State Ternary Complex, Ca2+ 手法: X-RAY DIFFRACTION / 解像度: 1.948 Å PDB-5txz: DNA Polymerase Mu Reactant Complex, 100mM Mg2+ (15 min) 手法: X-RAY DIFFRACTION / 解像度: 1.651 Å PDB-5tyb: DNA Polymerase Mu Reactant Complex, 10mM Mg2+ (7.5 min) 手法: X-RAY DIFFRACTION / 解像度: 1.848 Å PDB-5tyc: DNA Polymerase Mu Reactant Complex, 10mM Mg2+ (15 min) 手法: X-RAY DIFFRACTION / 解像度: 2.101 Å PDB-5tyd: DNA Polymerase Mu Reactant Complex, 10 mM Mg2+ (45 min) 手法: X-RAY DIFFRACTION / 解像度: 1.899 Å PDB-5tye: DNA Polymerase Mu Product Complex, 10 mM Mg2+ (60 min) 手法: X-RAY DIFFRACTION / 解像度: 2.047 Å PDB-5tyf: DNA Polymerase Mu Product Complex, 10 mM Mg2+ (270 min) 手法: X-RAY DIFFRACTION / 解像度: 1.971 Å PDB-5tyg: DNA Polymerase Mu Product Complex, 10 mM Mg2+ (960 min) 手法: X-RAY DIFFRACTION / 解像度: 1.726 Å PDB-5tyu: DNA Polymerase Mu Reactant Complex, Mn2+ (4 min) 手法: X-RAY DIFFRACTION / 解像度: 2.048 Å PDB-5tyv: DNA Polymerase Mu Reactant Complex, Mn2+ (7.5 min) 手法: X-RAY DIFFRACTION / 解像度: 1.93 Å PDB-5tyw: DNA Polymerase Mu Reactant Complex, Mn2+ (10 min) 手法: X-RAY DIFFRACTION / 解像度: 1.88 Å PDB-5tyx: DNA Polymerase Mu Product Complex, Mn2+ (15 min) 手法: X-RAY DIFFRACTION / 解像度: 1.948 Å PDB-5tyy: DNA Polymerase Mu Product Complex, Mn2+ (60 min) 手法: X-RAY DIFFRACTION / 解像度: 1.931 Å PDB-5tyz: DNA Polymerase Mu Product Complex, Mn2+ (960 min) 手法: X-RAY DIFFRACTION / 解像度: 1.977 Å |
---|
化合物 | ChemComp-EPE: 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / HEPES / pH緩衝剤*YM
|
---|
由来 | homo sapiens (ヒト)- unidentified (未定義)
|
---|
キーワード | TRANSFERASE/DNA / Time-Lapse Crystallography / Product Metal / DNA Polymerase Mu / Double Strand Break Repair / TRANSFERASE-DNA complex |
---|