EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation.
ジャーナル・号・ページ	Cell, Vol. 167, Issue 1, Page 133-144.e13, Year 2016
掲載日	2016年9月22日
著者	Tanweer Hussain / Jose L Llácer / Brian T Wimberly / Jeffrey S Kieft / V Ramakrishnan /
PubMed 要旨	In bacterial translational initiation, three initiation factors (IFs 1-3) enable the selection of initiator tRNA and the start codon in the P site of the 30S ribosomal subunit. Here, we report 11 ...In bacterial translational initiation, three initiation factors (IFs 1-3) enable the selection of initiator tRNA and the start codon in the P site of the 30S ribosomal subunit. Here, we report 11 single-particle cryo-electron microscopy (cryoEM) reconstructions of the complex of bacterial 30S subunit with initiator tRNA, mRNA, and IFs 1-3, representing different steps along the initiation pathway. IF1 provides key anchoring points for IF2 and IF3, thereby enhancing their activities. IF2 positions a domain in an extended conformation appropriate for capturing the formylmethionyl moiety charged on tRNA. IF3 and tRNA undergo large conformational changes to facilitate the accommodation of the formylmethionyl-tRNA (fMet-tRNA(fMet)) into the P site for start codon recognition.
リンク	Cell / PubMed:27662086 / PubMed Central
手法	EM (単粒子)
解像度	3.55 - 9.7 Å
構造データ	4073 5lmn EMDB-4073, PDB-5lmn: Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1A) 手法: EM (単粒子) / 解像度: 3.55 Å 4074 5lmo EMDB-4074, PDB-5lmo: Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1B) 手法: EM (単粒子) / 解像度: 4.3 Å 4075 5lmp EMDB-4075, PDB-5lmp: Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1C) 手法: EM (単粒子) / 解像度: 5.35 Å 4076 5lmq EMDB-4076, PDB-5lmq: Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex, open form (state-2A) 手法: EM (単粒子) / 解像度: 4.2 Å 4077 5lmr EMDB-4077, PDB-5lmr: Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-2B) 手法: EM (単粒子) / 解像度: 4.45 Å 4078 5lms EMDB-4078, PDB-5lms: Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-2C) 手法: EM (単粒子) / 解像度: 5.1 Å 4079 5lmt EMDB-4079, PDB-5lmt: Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-3) 手法: EM (単粒子) / 解像度: 4.15 Å 4080 5lmu EMDB-4080: Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex, closed form (state-4) PDB-5lmu: Structure of bacterial 30S-IF3-mRNA-tRNA translation pre-initiation complex, closed form (state-4) 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-4081: Structure of bacterial 30S-IF1-IF2-IF3-mRNA translation pre-initiation complex (state-I) 手法: EM (単粒子) / 解像度: 9.7 Å EMDB-4082: Structure of bacterial 30S-IF1-IF2-IF3-mRNA-tRNA translation pre-initiation complex(state-II) 手法: EM (単粒子) / 解像度: 8.3 Å 4083 5lmv EMDB-4083, PDB-5lmv: Structure of bacterial 30S-IF1-IF2-IF3-mRNA-tRNA translation pre-initiation complex(state-III) 手法: EM (単粒子) / 解像度: 4.9 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-A: ADENOSINE-5'-MONOPHOSPHATE / AMP ChemComp-G: GUANOSINE-5'-MONOPHOSPHATE / GMP ChemComp-U: URIDINE-5'-MONOPHOSPHATE / UMP ChemComp-FME: N-FORMYLMETHIONINE / N-ホルミルメチオニン
由来	thermus thermophilus hb8 (バクテリア) thermus thermophilus (バクテリア) thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (バクテリア) escherichia coli (大腸菌)
キーワード	RIBOSOME / translation / initiation factors / 30S / IF1 / IF3 / PIC / Thermus thermophilus / tRNAi / IF2