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-Structure paper
タイトル | Comprehensive molecular structure of the eukaryotic ribosome. |
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ジャーナル・号・ページ | Structure, Vol. 17, Issue 12, Page 1591-1604, Year 2009 |
掲載日 | 2009年12月9日 |
![]() | Derek J Taylor / Batsal Devkota / Andrew D Huang / Maya Topf / Eswar Narayanan / Andrej Sali / Stephen C Harvey / Joachim Frank / ![]() |
PubMed 要旨 | Despite the emergence of a large number of X-ray crystallographic models of the bacterial 70S ribosome over the past decade, an accurate atomic model of the eukaryotic 80S ribosome is still not ...Despite the emergence of a large number of X-ray crystallographic models of the bacterial 70S ribosome over the past decade, an accurate atomic model of the eukaryotic 80S ribosome is still not available. Eukaryotic ribosomes possess more ribosomal proteins and ribosomal RNA than do bacterial ribosomes, which are implicated in extraribosomal functions in the eukaryotic cells. By combining cryo-EM with RNA and protein homology modeling, we obtained an atomic model of the yeast 80S ribosome complete with all ribosomal RNA expansion segments and all ribosomal proteins for which a structural homolog can be identified. Mutation or deletion of 80S ribosomal proteins can abrogate maturation of the ribosome, leading to several human diseases. We have localized one such protein unique to eukaryotes, rpS19e, whose mutations are associated with Diamond-Blackfan anemia in humans. Additionally, we characterize crucial interactions between the dynamic stalk base of the ribosome with eukaryotic elongation factor 2. |
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手法 | EM (単粒子) |
解像度 | 8.9 Å |
構造データ | ![]() PDB-4v7h: |
由来 |
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![]() | RIBOSOME / eukaryotic ribosome / 80S / RACK1 protein / flexible fitting |