EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural characterization of mRNA-tRNA translocation intermediates.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 109, Issue 16, Page 6094-6099, Year 2012
掲載日	2012年4月17日
著者	Xabier Agirrezabala / Hstau Y Liao / Eduard Schreiner / Jie Fu / Rodrigo F Ortiz-Meoz / Klaus Schulten / Rachel Green / Joachim Frank /
PubMed 要旨	Cryo-EM analysis of a wild-type Escherichia coli pretranslocational sample has revealed the presence of previously unseen intermediate substates of the bacterial ribosome during the first phase of ...Cryo-EM analysis of a wild-type Escherichia coli pretranslocational sample has revealed the presence of previously unseen intermediate substates of the bacterial ribosome during the first phase of translocation, characterized by intermediate intersubunit rotations, L1 stalk positions, and tRNA configurations. Furthermore, we describe the domain rearrangements in quantitative terms, which has allowed us to characterize the processivity and coordination of the conformational reorganization of the ribosome, along with the associated changes in tRNA ribosome-binding configuration. The results are consistent with the view of the ribosome as a molecular machine employing Brownian motion to reach a functionally productive state via a series of substates with incremental changes in conformation.
リンク	Proc Natl Acad Sci U S A / PubMed:22467828 / PubMed Central
手法	EM (単粒子)
解像度	11.5 - 14.7 Å
構造データ	5359 4v6o EMDB-5359: New mRNA-tRNA translocation intermediates revealed by cryoEM: Class 4A (partially rotated (I) 70S ribosome with A-site and P-site tRNAs) PDB-4v6o: Structural characterization of mRNA-tRNA translocation intermediates (class 4a of the six classes) 手法: EM (単粒子) / 解像度: 14.7 Å 5360 4v6s EMDB-5360: New mRNA-tRNA translocation intermediates revealed by cryoEM: Class 3 (unrotated 70S ribosome with P-site tRNA) PDB-4v6s: Structural characterization of mRNA-tRNA translocation intermediates (class 3 of the six classes) 手法: EM (単粒子) / 解像度: 13.1 Å 5361 4v6n EMDB-5361: New mRNA-tRNA translocation intermediates revealed by cryoEM: Class 2 (unrotated 70S ribosome with A-site and P-site tRNAs) PDB-4v6n: Structural characterization of mRNA-tRNA translocation intermediates (50S ribosome of class2 of the six classes) 手法: EM (単粒子) / 解像度: 12.1 Å 5362 4v6r EMDB-5362: New mRNA-tRNA translocation intermediates revealed by cryoEM: Class 6 (rotated 70S ribosome with A-site and P-site tRNAs) PDB-4v6r: Structural characterization of mRNA-tRNA translocation intermediates (class 6 of the six classes) 手法: EM (単粒子) / 解像度: 11.5 Å 5363 4v6q EMDB-5363: New mRNA-tRNA translocation intermediates revealed by cryoEM: Class 5 (rotated 70S ribosome with A-site and P-site tRNAs) PDB-4v6q: Structural characterization of mRNA-tRNA translocation intermediates (class 5 of the six classes) 手法: EM (単粒子) / 解像度: 11.5 Å 5364 4v6p EMDB-5364: New mRNA-tRNA translocation intermediates revealed by cryoEM: Class 4B (partially rotated (II) 70S ribosome with A-site and P-site tRNAs) PDB-4v6p: Structural characterization of mRNA-tRNA translocation intermediates (class 4b of the six classes) 手法: EM (単粒子) / 解像度: 13.5 Å
化合物	ChemComp-FME: N-FORMYLMETHIONINE / N-ホルミルメチオニン ChemComp-TRP: TRYPTOPHAN / トリプトファン
由来	escherichia coli (大腸菌) sulfolobus acidocaldarius (好気性・好酸性)
キーワード	RIBOSOME / cryoelectron microscopy / molecular dynamics flexible fitting / ratchet-like rotation