EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Seeing tobacco mosaic virus through direct electron detectors.
ジャーナル・号・ページ	J Struct Biol, Vol. 189, Issue 2, Page 87-97, Year 2015
掲載日	2014年12月17日
著者	Simon A Fromm / Tanmay A M Bharat / Arjen J Jakobi / Wim J H Hagen / Carsten Sachse /
PubMed 要旨	With the introduction of direct electron detectors (DED) to the field of electron cryo-microscopy, a wave of atomic-resolution structures has become available. As the new detectors still require ...With the introduction of direct electron detectors (DED) to the field of electron cryo-microscopy, a wave of atomic-resolution structures has become available. As the new detectors still require comparative characterization, we have used tobacco mosaic virus (TMV) as a test specimen to study the quality of 3D image reconstructions from data recorded on the two direct electron detector cameras, K2 Summit and Falcon II. Using DED movie frames, we explored related image-processing aspects and compared the performance of micrograph-based and segment-based motion correction approaches. In addition, we investigated the effect of dose deposition on the atomic-resolution structure of TMV and show that radiation damage affects negative carboxyl chains first in a side-chain specific manner. Finally, using 450,000 asymmetric units and limiting the effects of radiation damage, we determined a high-resolution cryo-EM map at 3.35Å resolution. Here, we provide a comparative case study of highly ordered TMV recorded on different direct electron detectors to establish recording and processing conditions that enable structure determination up to 3.2Å in resolution using cryo-EM.
リンク	J Struct Biol / PubMed:25528571 / PubMed Central
手法	EM (らせん対称)
解像度	3.35 - 4.2 Å
構造データ	EMDB-2833: Cryo-EM structure of TMV reconstructed from micrographs recorded with the K2 Summit DED 手法: EM (らせん対称) / 解像度: 4.0 Å EMDB-2834: Cryo-EM structure of TMV reconstructed from micrographs recorded with the Falcon II DED 手法: EM (らせん対称) / 解像度: 3.7 Å EMDB-2835: Cryo-EM structure of TMV reconstructed from data ranging from 2.0-6.1 e-/A^2 accumulated dose 手法: EM (らせん対称) / 解像度: 3.5 Å EMDB-2836: Cryo-EM structure of TMV reconstructed from data ranging from 6.1-10.2 e-/A^2 accumulated dose 手法: EM (らせん対称) / 解像度: 3.5 Å EMDB-2837: Cryo-EM structure of TMV reconstructed from data ranging from 10.1-14.3 e-/A^2 accumulated dose 手法: EM (らせん対称) / 解像度: 3.5 Å EMDB-2838: Cryo-EM structure of TMV reconstructed from data ranging from 14.3-18.4 e-/A^2 accumulated dose 手法: EM (らせん対称) / 解像度: 3.6 Å EMDB-2839: Cryo-EM structure of TMV reconstructed from data ranging from 18.4-22.5 e-/A^2 accumulated dose 手法: EM (らせん対称) / 解像度: 3.8 Å EMDB-2840: Cryo-EM structure of TMV reconstructed from data ranging from 22.5-26.6 e-/A^2 accumulated dose 手法: EM (らせん対称) / 解像度: 4.1 Å EMDB-2841: Cryo-EM structure of TMV reconstructed from data ranging from 26.6-30.7 e-/A^2 accumulated dose 手法: EM (らせん対称) / 解像度: 4.2 Å 2842 4udv EMDB-2842, PDB-4udv: Cryo-EM structure of TMV at 3.35 A resolution 手法: EM (らせん対称) / 解像度: 3.35 Å
由来	tobacco mosaic virus (ウイルス)
キーワード	VIRAL PROTEIN / DIRECT ELECTRON DETECTORS / SINGLE PARTICLE HELICAL RECONSTRUCTION / HIGH RESOLUTION