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-Structure paper
タイトル | The Cryo-EM structure of the CorA channel from Methanocaldococcus jannaschii in low magnesium conditions. |
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ジャーナル・号・ページ | Biochim Biophys Acta, Vol. 1848, Issue 10 Pt A, Page 2206-2215, Year 2015 |
掲載日 | 2015年6月4日 |
著者 | Robert M Cleverley / James Kean / Chitra A Shintre / Clair Baldock / Jeremy P Derrick / Robert C Ford / Stephen M Prince / |
PubMed 要旨 | CorA channels are responsible for the uptake of essential magnesium ions by bacteria. X-ray crystal structures have been resolved for two full-length CorA channels, each in a non-conducting state ...CorA channels are responsible for the uptake of essential magnesium ions by bacteria. X-ray crystal structures have been resolved for two full-length CorA channels, each in a non-conducting state with magnesium ions bound to the protein: These structures reveal a homo-pentameric quaternary structure with approximate 5-fold rotational symmetry about a central pore axis. We report the structure of the detergent solubilized Methanocaldococcus jannaschii CorA channel determined by Cryo-Electron Microscopy and Single Particle Averaging, supported by Small Angle X-ray Scattering and X-ray crystallography. This structure also shows a pentameric channel but with a highly asymmetric domain structure. The asymmetry of the domains includes differential separations between the trans-membrane segments, which reflects mechanical coupling of the cytoplasmic domain to the trans-membrane domain. This structure therefore reveals an important aspect of the gating mechanism of CorA channels by providing an indication of how the absence of magnesium ions leads to major structural changes. |
リンク | Biochim Biophys Acta / PubMed:26051127 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 21.6 Å |
構造データ | |
由来 |
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キーワード | MEMBRANE PROTEIN / MAGNESIUM ION CHANNEL |