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-Structure paper
タイトル | Structural changes in dengue virus when exposed to a temperature of 37°C. |
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ジャーナル・号・ページ | J Virol, Vol. 87, Issue 13, Page 7585-7592, Year 2013 |
掲載日 | 2013年5月1日 |
著者 | Guntur Fibriansah / Thiam-Seng Ng / Victor A Kostyuchenko / Jaime Lee / Sumarlin Lee / Jiaqi Wang / Shee-Mei Lok / |
PubMed 要旨 | Previous binding studies of antibodies that recognized a partially or fully hidden epitope suggest that insect cell-derived dengue virus undergoes structural changes at an elevated temperature. This ...Previous binding studies of antibodies that recognized a partially or fully hidden epitope suggest that insect cell-derived dengue virus undergoes structural changes at an elevated temperature. This was confirmed by our cryo-electron microscopy images of dengue virus incubated at 37°C, where viruses change their surface from smooth to rough. Here we present the cryo-electron microscopy structures of dengue virus at 37°C. Image analysis showed four classes of particles. The three-dimensional (3D) map of one of these classes, representing half of the imaged virus population, shows that the E protein shell has expanded and there is a hole at the 3-fold vertices. Fitting E protein structures into the map suggests that all of the interdimeric and some intradimeric E protein interactions are weakened. The accessibility of some previously found cryptic epitopes on this class of particles is discussed. |
リンク | J Virol / PubMed:23637405 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 13.7 Å |
構造データ | |
由来 |
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キーワード | VIRUS / BREATHING STRUCTURE / DYNAMIC STRUCTURE |