EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The molecular architecture of the bacteriophage T4 neck.
ジャーナル・号・ページ	J Mol Biol, Vol. 425, Issue 10, Page 1731-1744, Year 2013
掲載日	2013年5月27日
著者	Andrei Fokine / Zhihong Zhang / Shuji Kanamaru / Valorie D Bowman / Anastasia A Aksyuk / Fumio Arisaka / Venigalla B Rao / Michael G Rossmann /
PubMed 要旨	A hexamer of the bacteriophage T4 tail terminator protein, gp15, attaches to the top of the phage tail stabilizing the contractile sheath and forming the interface for binding of the independently ...A hexamer of the bacteriophage T4 tail terminator protein, gp15, attaches to the top of the phage tail stabilizing the contractile sheath and forming the interface for binding of the independently assembled head. Here we report the crystal structure of the gp15 hexamer, describe its interactions in T4 virions that have either an extended tail or a contracted tail, and discuss its structural relationship to other phage proteins. The neck of T4 virions is decorated by the "collar" and "whiskers", made of fibritin molecules. Fibritin acts as a chaperone helping to attach the long tail fibers to the virus during the assembly process. The collar and whiskers are environment-sensing devices, regulating the retraction of the long tail fibers under unfavorable conditions, thus preventing infection. Cryo-electron microscopy analysis suggests that twelve fibritin molecules attach to the phage neck with six molecules forming the collar and six molecules forming the whiskers.
リンク	J Mol Biol / PubMed:23434847 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.7001 - 25.0 Å
構造データ	5528 3j2o EMDB-5528: Cryo-EM structure of the contracted bacteriophage T4 tail containing the collar and whiskers made of fibritin molecules. PDB-3j2o: Model of the bacteriophage T4 fibritin based on the cryo-EM reconstruction of the contracted T4 tail containing the phage collar and whiskers 手法: EM (単粒子) / 解像度: 25.0 Å PDB-3j2m: The X-ray structure of the gp15 hexamer and the model of the gp18 protein fitted into the cryo-EM reconstruction of the extended T4 tail 手法: ELECTRON MICROSCOPY / 解像度: 15.0 Å PDB-3j2n: The X-ray structure of the gp15 hexamer and the model of the gp18 protein fitted into the cryo-EM reconstruction of the contracted T4 tail 手法: ELECTRON MICROSCOPY / 解像度: 16.0 Å PDB-4hud: Structure of the bacteriophage T4 tail terminator protein, gp15. 手法: X-RAY DIFFRACTION / 解像度: 2.7001 Å PDB-4huh: Structure of the bacteriophage T4 tail terminator protein, gp15 (C-terminal truncation mutant 1-261). 手法: X-RAY DIFFRACTION / 解像度: 3.2 Å
化合物	ChemComp-HOH: WATER / 水
由来	enterobacteria phage t4 (ファージ)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / bacteriophage T4 (T4ファージ) / phage tail terminator protein / phage sheath protein / phage neck / collar whiskers / fibritin / gpwac / gp15