タイトル | Probing the substrate specificity of Golgi alpha-mannosidase II by use of synthetic oligosaccharides and a catalytic nucleophile mutant. |
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ジャーナル・号・ページ | J. Am. Chem. Soc., Vol. 130, Page 8975-8983, Year 2008 |
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掲載日 | 2008年1月2日 (構造データの登録日) |
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著者 | Zhong, W. / Kuntz, D.A. / Ember, B. / Singh, H. / Moremen, K.W. / Rose, D.R. / Boons, G.J. |
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リンク | J. Am. Chem. Soc. / PubMed:18558690 |
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手法 | X線回折 |
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解像度 | 1.1 - 2.03 Å |
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構造データ | PDB-3bub: Golgi alpha-mannosidase II with an empty active site 手法: X-RAY DIFFRACTION / 解像度: 1.38 Å PDB-3bud: Golgi mannosidase II D204A catalytic nucleophile mutant with an empty active site 手法: X-RAY DIFFRACTION / 解像度: 1.85 Å PDB-3bui: Golgi mannosidase II D204A catalytic nucleophile mutant complex with Tris 手法: X-RAY DIFFRACTION / 解像度: 1.25 Å PDB-3bup: Golgi alpha-mannosidase II D341N acid-base catalyst mutant with bound mannose 手法: X-RAY DIFFRACTION / 解像度: 2.03 Å PDB-3buq: Golgi alpha-mannosidase II D204A catalytic nucleophile mutant with bound mannose. 手法: X-RAY DIFFRACTION / 解像度: 2.01 Å PDB-3bvt: GOLGI MANNOSIDASE II D204A catalytic nucleophile mutant complex with Methyl (alpha-D-mannopyranosyl)-(1->3)-S-alpha-D-mannopyranoside 手法: X-RAY DIFFRACTION / 解像度: 1.3 Å PDB-3bvu: GOLGI MANNOSIDASE II D204A catalytic nucleophile mutant complex with Methyl(alpha-D-mannopyranosyl)-(1->3)-S-[(alpha-D-mannopyranosyl)-(1->6)]-alpha-D-mannopyranoside 手法: X-RAY DIFFRACTION / 解像度: 1.12 Å PDB-3bvv: Golgi mannosidase II D204A catalytic nucleophile mutant complex with METHYL ALPHA-D-MANNOPYRANOSYL-(1->3)-[6-THIO-ALPHA-D-MANNOPYRANOSYL-(1->6)]-BETA-D-MANNOPYRANOSIDE 手法: X-RAY DIFFRACTION / 解像度: 1.3 Å PDB-3bvw: GOLGI MANNOSIDASE II D204A catalytic nucleophile mutant complex with Methyl (2-deoxy-2-acetamido-beta-D-glucopyranosyl)-(1->2)-ALPHA-D-mannopyranosyl- (1->3)-[ALPHA-D-mannopyranosyl-(1->6)-6-thio-alpha-D-mannopyranosyl- (1->6)]-BETA-D-mannopyranoside 手法: X-RAY DIFFRACTION / 解像度: 1.2 Å PDB-3bvx: GOLGI MANNOSIDASE II D204A catalytic nucleophile mutant complex with Methyl (2-deoxy-2-acetamido-beta-D-glucopyranosyl)-(1->2)-(alpha-D-mannopyranosyl)- (1->3)-[(alpha-D-mannopyranosyl)-(1->6)-(alpha-D-mannopyranosyl)-(1->6)]-beta-D-mannopyranoside 手法: X-RAY DIFFRACTION / 解像度: 1.1 Å |
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化合物 | |
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由来 | - drosophila melanogaster (キイロショウジョウバエ)
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キーワード | HYDROLASE / GLYCOSYL HYDROLASE FAMILY 38 / Glycosidase / Golgi apparatus / Membrane / Signal-anchor / Transmembrane / FAMILY 38 GLYCOYSL HYDROLASE |
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