| タイトル | Catalytic mechanism of nitrile hydratase proposed by time-resolved X-ray crystallography using a novel substrate, tert-butylisonitrile |
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| ジャーナル・号・ページ | J. Biol. Chem., Vol. 283, Page 36617-36623, Year 2008 |
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| 掲載日 | 2008年7月9日 (構造データの登録日) |
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著者 | Hashimoto, K. / Suzuki, H. / Taniguchi, K. / Noguchi, T. / Yohda, M. / Odaka, M. |
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リンク | J. Biol. Chem. / PubMed:18948265 |
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| 手法 | X線回折 |
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| 解像度 | 1.3 - 1.59 Å |
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| 構造データ | PDB-2zpb: nitrosylated Fe-type nitrile hydratase 手法: X-RAY DIFFRACTION / 解像度: 1.3 Å PDB-2zpe: nitrosylated Fe-type nitrile hydratase with tert-butylisonitrile 手法: X-RAY DIFFRACTION / 解像度: 1.48 Å PDB-2zpf: Complex of Fe-type nitrile hydratase with tert-butylisonitrile, photo-activated for 18min at 293K 手法: X-RAY DIFFRACTION / 解像度: 1.482 Å PDB-2zpg: Complex of Fe-type nitrile hydratase with tert-butylisonitrile, photo-activated for 120min at 293K 手法: X-RAY DIFFRACTION / 解像度: 1.39 Å PDB-2zph: Complex of Fe-type nitrile hydratase with tert-butylisonitrile, photo-activated for 340min at 293K 手法: X-RAY DIFFRACTION / 解像度: 1.59 Å PDB-2zpi: Complex of Fe-type nitrile hydratase with tert-butylisonitrile, photo-activated for 440min at 293K 手法: X-RAY DIFFRACTION / 解像度: 1.491 Å |
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| 化合物 | ChemComp-TRS: 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH緩衝剤*YM
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| 由来 | rhodococcus erythropolis (バクテリア)
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キーワード | LYASE / Iron / Metal-binding / Oxidation |
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